碩士 / 國立中山大學 / 海洋資源研究所 / 86 / The studies forcus on spermiogenesis and capacitation of sperm of tiger prawn, ~u2;Penaeus~u1; ~u2;monodon~u1;. After spermatogenesis of the sperm has finished in the testis, spermiogenesis are in progess in proximal vas deferens. The major change in the sperm during spermiogenesis is the extension of the spike. Sperm progessively extend its single spike from the front of the round main body. The growth of the spike finish when spermatids move into medial vas deferens ascending limb. There is no difference in spike length between the sperm in medial vas deferens limb and spermatophore. We studies sperm in male vas deferens (VD), spermatophore (SPH) and female seminal receptacle (SR) of the tiger prawn for understanding the capacitation of the sperm in this shrimp specues. During mating, spermatophores of male tiger prawn are transfered by petasma at the base of first pair of pleopods to female thelycum between fourth and fifth pair of pereipods. The sperm mass mix with the secretion of the thelycum and store in exoskeletal culticle seminal receptacles. Using egg water (EW) to induce sperm acitvation to examine the degree od sperm capacitation in different region of the reproduction tract. The results reveal that tiger prawn do proceed capacitation in the thelycum since the sperm from SR reacted to EW while the sperm from VD and SPH do not reacted to EW. We use spermatophore transplatation to artificially transfer the SPH to female thelycum to examine ~u2;in~u1; ~u2;vivo~u1; capacitation process in the tiger prawn. The results showes that more than 10% sperm are able to exocytosis induced by EW after 6 hr transplantation; more than 40% sperm are capable to be induced exocytosis by EW after 24 hr transplantation. Sperm isolated from medial vas deferens ascending limb and spermatophore were incubated in different pH artificial sea water (ASW), seminal fluid, male hemolymph and female hemolymph to process ~u2;in~u1; ~u2;vitro~u1; capacitation. The percentage of sperm exocytosis induced by EW were much higher in sperm being incubated in higher pH (pH8.0-9.0) than in lower pH (pH5.0-7.0) ASW. Sperm incubated in high pH ASW with the addition of seminal fluid had higher sperm exocytosis rate than incubated in same pH ASW. The results indicated that seminal fluid can promote the efficiency of inducing sperm capacitation in high pH ASW ~u2;in~u1; ~u2;vitro~u1;.-1 -aThe studies of morphology and physiology of sperm maturation in tiger prawn, ~u2;Penaeus~u1; ~u2;monodon~u1
| Identifer | oai:union.ndltd.org:TW/086NSYSU277006 |
| Date | January 1998 |
| Creators | Kuan,Chien-Chuan, 官建權 |
| Contributors | Chen,Tzyy-Ing, Chen,Meng-Hsien, 陳紫瑛, 陳孟仙 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 93 |
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