碩士 / 國立海洋大學 / 水產生物技術研究所 / 86 / Superoxide dismutases (SODs) are metalloenzymes that detoxify superoxide radicals present in various living cells. A gene encoding manganese superoxide dismutase (Mn-SOD) was cloned successfully from Vibrio mimicus by both traditional and random primer gene walking PCR which using the combination of degenerate and gene specific primers. The full-length Mn-SOD gene was composed of 606 base pair and encoded a 202 amino acid open reading frame.The amino acid sequence exhibits a 56.9% indentity to the Mn-SOD from Bacillus caldotenax and shows an overall homology to sequences of other known Mn-SODs. The V. mimicus Mn-SOD gene was introduced into the pET20b(+) expression vector and then transformed into the E. coli BL21 (DE3) pLysS expression host for the heterologous gens expression. The specificity of V. mimicus Mn-SOD gene probe was analyzed by PCR among several Vibrio spp. tested in order to provide the preliminary information for the purpose of Vibrio strain identification.
| Identifer | oai:union.ndltd.org:TW/086NTOU3613006 |
| Date | January 1998 |
| Creators | 金志宇 |
| Contributors | 林富邦 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 85 |
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