碩士 / 國立臺灣大學 / 漁業科學研究所 / 86 / Calcium ion plays an important role for larvae development. The present study,
using different calcium concentrations as environmental fators, investigated
the calcium balance mechanism in developing tilapia larvae. The abilities to
modulate calcium influx in larvae varied with different acculimation periods
and developmental stages. In the artificial freshwater with 0.2 mM [Ca2+],
tilapia larvae showed the highest calcium influx rate 3 d after hatching, and
this was associated with the proliferation of gills which occurred at the same
time. Among different stage, 3-d-old larvae showed the highest degree of
decrease in calcium influx and in calcium content upon transfer to low-calcium
medium. These results suggest that 3 d after hatching is the most sensitive
to environmental changes. Tilapia larva was acclimated at different calcium
concentration including, high calcium ([Ca2+]=2 mM), normal calcium ([Ca2+]=
0.2 mM), and low calcium ([Ca2+=0.02 mM]). Calcium influx in low-calcium
larvae was lower than that in high-calcium larvae during 24-96 h after hatc
hi-ng, but the former turned out to be higher than the latter 120 h after hatc
hi-ng. The calcium content in low-calcium larvae was lower than that in high-
calcium larvae throughout whole experiment. These results suggest that increa-
sing calcium influx is a compensation for decreased calcium content in the
larvae adapted to low-calcium environment. Moreover, this adaptation is dev-elo
ped as early as 24 h after hatching, and is does (environmental calcium con-
centration) related. Using Western blotting both Ca2+-ATPase-like and calbi di
n-D2k-like were found to express in gills and yolk-sac epithelia of tilapia la
rvae. 0-7 d after hatching, suggesting these transport protein are involved in
the calcium balance mechanism of developing larvae.
| Identifer | oai:union.ndltd.org:TW/086NTU00451009 |
| Date | January 1998 |
| Creators | Lu, Fu-I, 盧福翊 |
| Contributors | Huang Pung-Pung, Lee Ying-Chou, 黃鵬鵬, 李英周, --- |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 58 |
Page generated in 0.0014 seconds