碩士 / 國立交通大學 / 環境工程所 / 87 / In this study , we performed the modified method of the continuous algal(Selenastrum capricornutum) toxicity test which was introduced by Chen & Lin in 1996 . By diluting , we decreased the use of algal cells per ml from 100 % to 50 %、6 % in each continuous experiment . We then researched the correlation between the algal cell density with the sensitivity of the continuous toxicity test . And we found that : When we lowered the algal cell density , the sensibility would be improved markedly i.e. the EC50 values 、NOEC values and LOEC values dropped off . In addition , the lower cell density could shorten the experimental period from 4 days to two or one day . The short period could increase the frequency of the operation of the experiment .
Because the new algal standard batch method is intend to inhibit the use of the chelator-EDTA in the medium , we also proceeded the correlative experiments which not including (or reduced) EDTA . Then , we researched how the lack of EDTA could effect on the algal growth , and compared the results of the toxicity test from the exist of EDTA or not . Additionally , we proceeded the batch toxicity test without EDTA in comparison to the results from continuous toxicity test. Then we found that : when the EDTA , in the medium , was reduced from 30 μg/L to 15 μg/L , the culture of algae in the continuous incubator was still maintained in healthy condition for a long time . In the toxicity test , however, when we reduced the use of the EDTA , the sensibility would be increased . Finally , we compared the reproducibility in each different toxicity test .
| Identifer | oai:union.ndltd.org:TW/087NCTU0515020 |
| Date | January 1999 |
| Creators | Yi-Da Chen, 陳怡達 |
| Contributors | Chang-Yuan Chen, 陳重元 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 101 |
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