碩士 / 國立海洋大學 / 食品科學系 / 87 / Changes in viable bacterial cell number, cell morphology and hydrophobicity during starvation were determined with a mucoid Vibrio alginolyticus HT01 isolated from Patsu Mesn pier, Keelung, Taiwan. Cell adhesion on solid surface were determined with four flow rates in a tunnel circulation flow system. The fine structure of cell adhesion on solid surface was determined by both scanning electron microscopy and transmitting electron microscopy. Rods cells in normal culture condition was changed to sphere cells under starvation. A normal viable cell count of 108 CFU/ml was reduced to 106 CFU/ml when cells entered in starvation and the cell density of OD650 was decreased accordingly. Cells at 46 days starvation appeared as spheres or rods and still possessed monotrichous flagellum. A mucoid substance surrounded the cell appeared and the cell number was reduced from 108 to 104 CFU/ml. After temperature up-shift for 3 days the starved cells were resuscinated to reach 108 CFU/ml again. Changes in hydrophobicity of starved cells were determined and found decreased when measured with both bacterial adhesion hrdrocarbon (BATH) and hydrophobic interaction chromatography (HIC) methods.
Cell adhesion on both coverglass and polyester disks with four different flow rates were determined. Adhesion of normal cells on coverglass was found highest at 36 h adhesion with both 0.38 and 0.65 km/h flow rates among the four different flow rates determined. For the adhesion on polyester disks, flow rate at 0.38 km/h was also found highest than the other three flow rates. A layer of extracellular polysaccharides was appeared when cell adhesion on both coverglass and polyester disks was examined with SEM. Cell morphology was graduately changed from rods to spheres and even intent shapes as adhesion process prolonged. Cells aderent to coverglass were not varied with changes in flow rate in one week starved cells. These one week starved cells had a similar adhesion rate on coverglass similar to that of normal cells as more cells became sphere when adhesion time was extended. It was not found in the case of adhesion on polyester disks. Nevertheless, more extracellular polysaccharide substance was found on polyester disks than on coveglass. Cell adhesion for two week starved cells was similar to that of one week starved cells. Under SEM observation cells adhesion of two week starvation cells to both coverglass and polyester disks were similar to that of one week starvation cells. But, more extracellular polysaccharides were evidently increased.
| Identifer | oai:union.ndltd.org:TW/087NTOU0253029 |
| Date | January 1999 |
| Creators | Ling-Ling Liu, 劉玲伶 |
| Contributors | T. J. Chai, 蔡土及 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 107 |
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