碩士 / 國立海洋大學 / 海洋生物研究所 / 87 / Abstract
DNA polymerase α and proliferating cell nuclear antigen (PCNA) are two DNA replication-related genes. The main purpose of this is to distinguish which gene is suitable for the application in estimation of phytoplankton population growth rate. A diatom (Skeletonema costatum) is used in this study. The mRNA content of these 2 genes was detected by competitive quantitative reverse transcription-polymerase chain reaction (CQ-RT-PCR) at various growth stages and different UV-treatment conditions. During exponential phase, population growth rate reached 0.6 day-1. The level of DNA polymerase α mRNA ranging from 600 to 1200 molecules cell-1 detected in cells from exponential phase, is higher than the level of 0 to 500 molecules cell-1 found in cells from the stationary phase. Similarly, PCNA mRNA level was between 900 and 3500 molecules cell-1 during exponential phase but decreased to 0 to 500 molecules cell-1 in stationary phase. In UV treatment experiments, DNA of S. costatum was severely damaged by UVC treatment and a large quantity of thymidine-dimers was formed. The mRNA concentration of DNA polymerase α and PCNA were high in the UVC-treated samples. The results indicate that both DNA polymerase α and PCNA were involved in repair of DNA damage. In UVA/B treatment, T-dimer formation was less obvious mRNA concentration of DNA polymerase α and PCNA were similar in both UVA/B-treated and control samples. In contrast, the level of PCNA protein seemed to increase in UVA/B-treated samples. Since UVA/B is the major form of UV radiation in the natural environment, our results suggest that both mRNA of DNA polymerase α and PCNA are suitable indicators for estimation of population growth rate of S. costatum.
| Identifer | oai:union.ndltd.org:TW/087NTOU0270009 |
| Date | January 1999 |
| Creators | Wei Su Fen, 魏蜀芬 |
| Contributors | Jang Cheng, S.-P. L. Hwang, 張正, 黃聲蘋 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 75 |
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