碩士 / 國立海洋大學 / 水產生物技術研究所 / 87 / The superoxide dismutase gene from Vibrio metschnikovii was cloned successfully by PCR techniques using the degenerate Mn-SOD No and Mn-SOD Co primers, and the random primer gene walking PCR using V. metschnikovii sod gene specific primers and random hexamer. The full-length sod gene was composed of 588 base pair and encoded 196 amino acid residues. Its amino acid sequence showed high homology to Photobacterium leiognathi, E. coli Fe-sod gene (85.5%, 76.2%, respectively). V. metschnikovii sod gene was cloned into the expression vector pET20b(+) and expressed in E. coli BL21(DE3)pLysS. Biochemical characteristics of the purified recombinant Vme-SOD have confirmed it encoded a Fe- or Mn-SOD. The specificity of V. metschnikovii sod gene probe was analyzed by cross-species PCR in order to provide the preliminary identification of Vibrio strains.
| Identifer | oai:union.ndltd.org:TW/087NTOU0613004 |
| Date | January 1999 |
| Creators | ma hsiao fen, 馬小芬 |
| Contributors | lin fu pang, 林富邦 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 81 |
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