碩士 / 國立臺灣大學 / 動物學研究所 / 87 / Carp elongation factor 1α( EF-1α) and ribosomal proteins( L37 and S17) were isolated from an ovary cDNA library. EF-1α is a 1651bp cDNA and encodes a 458 residue polypeptide. L37 is a 389bp cDNA and encodes 92 residue polypeptide. S17 is a 434bp cDNA and encodes 135 residue polypeptide. They are extremely high homologous to their counterparts in other animals. In this paper, I study the expression change of EF-1α, L37 and S17 during the oogenesis to understand the translation during development of carp oocyte.
Northern blotting revealed that EF-1α, L37 and S17 mRNA reach the highest level in the second stage of oogenesis, but they decrease with the oocyte maturation. Western blot analysis indicated that EF-1αprotein is accumulated until the second stage and then decreases. However, it failed to detect L37 and S17 proteins in oocytes by antiserum against bacterial recombinant L37 and S17 proteins.
| Identifer | oai:union.ndltd.org:TW/087NTU00312016 |
| Date | January 1999 |
| Creators | Ts'ai-Lien Liao, 廖彩蓮 |
| Contributors | Fore-Lien Huang, Yea-Sha Chang, 黃火鍊, 張月霞 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 64 |
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