碩士 / 國立臺灣大學 / 植物學研究所 / 87 / Cyanidium caldarium live in extreme environments about 50℃ and pH 2. It's a primitive eucaryote from observation using transmission-electron microscope. The alga has a nucleus and a big chloroplast which cell diameter is about 4 μm.
The algas are treated at 48℃ as heat shock and those are cultured at room temperature as control, using 35S-methionine to label their proteins. After running SDS-polyacrylamide gel electrophoresis and autoradiography, some proteins are synthesized and accumulated. Their molucular weight are 97 KDa, 60 KDa, 39 Kda, 36 KDa, 32 KDa, 23 KDa, 22 KDa, 16 KDa and 15 KDa. We use E. coli GroEL antibody, yeast HSP60 antibody and soybean class I low-molecular-weight heat shock proteins antibody to detect this alga's proteins. From result on immunoblotting, the red alga's proteins don't react with E. coli GroEL antibody and soybean class I LMW antibody, but yeast HSP60 antibody have cross-reaction with C. caldarium, The reactive proteins amount accumulate as increasing period of heat shock treatment. So C. caldarium have high homologous with yeast than with E. coli or soybean.
After extracting C. caldarium genomic DNA and digested by BamHI and HindIII, we use yeast hsp60 cDNA fragment as probe for Southern hybridization. There are two hybrid bands in DNA, digested by BamHI, and their size are 2.1and 1.7 Kb, marked as B2.1 and B1.7. Only one hybrid band appear in DNA digested by HindIII, fragment length is 2.7 Kb, marked as H2.7.
| Identifer | oai:union.ndltd.org:TW/087NTU00366006 |
| Date | January 1999 |
| Creators | Jung,zu-wen, 江志文 |
| Contributors | Chen,Yih-ming, 陳益明 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 62 |
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