碩士 / 國立臺灣大學 / 農業化學研究所 / 87 / Abstract
Four isolates of Chlorella (Chlorella sp. strains I, V, 21, 216), green algae, were isolated from ponds and rivers in Taiwan and Indonesia. These strains were proven on a preliminary basis to be potential mass culturing strains. All cells are 5-10 mm in diameter, and cells are spherical in shape. All mature vegetative cells contained pyrenoid, and were surrounded by two starch grains forming the cup-shaped entities under chloroplasts. All four strains of Chlorella sp. can form autospores for their asexual reproduction and 2-4 autospores were usually observed from one mother cell.
The taxonomy of the unicellular alga Chlorella is very difficult because of its morphological simplicity. Only a few taxonomical studies have been based on morphology. Chlorella species were classified into two groups by the sugar composition of the cell wall. One group had a rigid cell wall composed of glucose and mannose (glucan type), and the other had a rigid cell wall composed of glucosamine (glucosamine type). The structure of the pyrenoid supports the separation of Chlorella species into two groups based on cell wall chemistry and suggests evolutionary relationships. Chlorella species with a glucan-type wall exhibit quite diverse pyrenoid structures, but those species with a glucosamine-type wall (C. kessleri, C. sorokiniana and C. vulgaris) are virtually identical in pyrenoid morphology, in which the thylakoid that penetrates into the pyrenoid matrix, is uniformly double-layered, indicating closer evolutionary relationships. Additional characters of lactose and sucrose fermentation, Chlorella sp. strains I, V, 21, 216 is closer to C. sorokiniana and C. vulgaris.
We have examined the pigments of Chlorella sp. I, V, 21, 216. For the pigment composition, all cells consist of chlorophyll a & b, violaxanthin and lutein. In dry spray powder form, the photoprotective xanthophyll cycle could be found and it moved in the direction of de-epoxidation. The concentration of violaxanthin decreased, but the concentration of lutein increased。Because chlorophyllide and phyto groups were esterificated the concentration of chlorophylls increased .
| Identifer | oai:union.ndltd.org:TW/087NTU00406050 |
| Date | January 1999 |
| Creators | Chia-Fang Wu, 吳佳芳 |
| Contributors | Liang-Ping Lin, 林良平 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 0 |
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