碩士 / 國立臺灣大學 / 漁業科學研究所 / 87 / Using rapid amplification of cDNA ends (RACE), full length cDNAs of Na,K-ATPase α1 and α3 subunits of tilapia (Oreochromis mossambicus) were cloned and sequenced. Clone TG33 is 3390 bp in length and encodes a polypeptide of 1023 amino acids, while clone TH3 is 3581 bp in length and encodes a polypeptide of 1010 amino acids. Clones TG33 and TH3 showed 91 ％ and 88 ％ identity at amino acid level with previously described animal Na,K-ATPase α1 and α3 subunits, respectively. Northern blot analysis showed that α1 subunit is predominantly expressed in kidney and intestine and α3 subunits is expressed in brain and heart. Results of immunoblotting and RT-PCR indicate that Na,K-ATPase α1 subunit is ubiquitously expressed. The amount of α1 subunit mRNA and protein in gill tissue increased with the level of environmental salinity. Our results indicate that the salinity-dependent stimulation of mRNA expression of gill Na,K-ATPase α1 subunit is associated with corresponding stimulation at the protein level. This provides direct evidence of enhanced transcription and translation of Na,K-ATPase α1 subunit gene upon salinity challenge.
| Identifer | oai:union.ndltd.org:TW/087NTU00451002 |
| Date | January 1999 |
| Creators | Hsin-Hui Feng, 馮馨慧 |
| Contributors | Pung-Pung Hwang, 黃鵬鵬 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 90 |
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