碩士 / 國立臺灣大學 / 藥理學研究所 / 87 / Abstract
The effects of arecoline on stimulus-evoked population spike of rat hippocampus were investigated in vitro. Arecoline (0.01-10 microM) reversibly decreased the amplitudes of CA1 evoked population spikes in a concentration dependent manner. Atropine (3 microM) significantly prevented the arecoline (10 microM) elicited decreasing effects of the population spike amplitudes but d-tubocurarine (1 microM) did not alter the decreaseing effects of arecoline. Arecoline decreased the amplitudes of population spikes even after pretreatment of pirenzepine (M1 muscarinic receptor antagonist, 0.1 microM), methoctramine (M2 muscarinic receptor antagonist, 0.25 microM) and tropicamide (M4 muscarinic receptor antagonist, 1 microM). However, arecoline did not decrease the amplitudes of population spikes if the M3 muscarinic receptor antagonist 4-diphenylacetoxy-N-methylpiperidine methiodide (4-DAMP, 1 microM) was pretreated. Arecoline (1-10 microM) significantly increased the ratio of the second pulse response to the first pulse response (P2/P1), which indicated a decrease in the transmitter release probability. The M3 muscarinic receptor antagonist, 4-DAMP (1 microM) can block this effect. These results suggest that arecoline could mediate the depression of population spikes by M3 receptor.
| Identifer | oai:union.ndltd.org:TW/087NTU01550016 |
| Date | January 1999 |
| Creators | Chen-Ming Teng, 鄧振銘 |
| Contributors | Ming-Cheng Tsai, 蔡明正 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 77 |
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