碩士 / 淡江大學 / 水資源及環境工程學系 / 87 / It’s well known that mercury enters the environment through a number of natural and industrial processes and is found in several chemical forms. Because many microorganisms exhibit the ability to methylate the inorganic mercury. The Large amount of methylmercury, the most toxic species among them, has been found in fish.
A simple and rapid method is described for the extraction and determination of methylmercury in fish. The procedure was mainly based on the quantitative microwave-assisted extraction of methylmercury from fish sample using toluene as extract solvent.
Taguchi's orthogonal array design (L9) have been used to optimize the microwave extraction process. Factors such as temperature, microwave power, extraction time and solvent volume were considered. The results suggest that all four factors are significantly. The optimize microwave process was 120˚C, 15 mL Toluene, 5 minutes extraction time, and 100%(947W) microwave power.
The analysis of extracts has been carried out by capillary gas chromatography with electronic capture detector. The detection limit of the procedure was 10 pg/μL. And the methylmercury in marine fish such as tuna, swordfish, shark were found 0.68, 0.75, 0.40 μg/g respectively and the river fish such as Tilapia wasn’t found any methylmercury. Validation was carried out by certified reference material(DORM-2) of National Research Concil in Canada, and the recovery was more than 98%.
| Identifer | oai:union.ndltd.org:TW/087TKU00088001 |
| Date | January 1999 |
| Creators | Cheng-Ping Shin, 施政平 |
| Contributors | Tau-Being Hsu, 許道平 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 105 |
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