碩士 / 國立中興大學 / 獸醫微生物學研究所 / 88 / The aims of this instigation are to study the effect of five disinfectants, iodorin-200, caflon BKC 80, formalin, malachite green, and SCI-62, on the Staphylococcus aureas subsp. aureus., Streptococcus sp., Aeromonas hydrophila, Edwardsiella tarda, Escherichia coli, Pseudomonas anguilliseptica, and Vibrio anguillarum from the soft-shell turtle and tilpia . At the same time, the acute toxicities of the above disinfectants to the soft-shell turtle and tilapia were studied.
Our sesults indicated that the phenol coefficients of iodorin-200 on Staphylococcus aureas subsp. aureus., Streptococcus sp. Aeromonas hydrophila, Edwardsiella tarda, Escherichia coli, Pseudomonas anguilliseptica, and Vibrio anguillarum were 0.68 ± 0.07, 0.90 ± 0.05, 0.74 ± 0.04, 0.81 ± 0.05, 0.71 ± 0.04, 0.68 ± 0.04, and 0.80 ± 0.04, respectively. Those of caflon BKC 80 were 160.7 ± 6.5, 224.6 ± 9.0, 223.0 ± 11.5, 227.8 ± 13.4, 132.6 ± 5.2, 246.5 ± 6.3, and 214.8 ± 8.0, respectively. The coefficients of formalin were 1.46 ± 0.07, 1.21 ± 0.01, 1.40 ± 0.04, 1.42 ± 0.05, 1.37 ± 0.06, 1.49 ± 0.11, and 1.25 ± 0.05, respectively. Those of malachite green were 64.5 ± 2.7, 21.0 ± 2.0, 20.8 ± 2.7, 20.8 ± 2.7, 15.8 ± 0.7, 16.3 ± 2.6, and 16.0 ± 2.3, respectively. The coefficients of SCI-62 were 0.20 ± 0.04, 0.18 ± 0.04, 0.14 ± 0.01, 0.14 ± 0.05, 0.11 ± 0.05, 0.17 ± 0.05, and 0.10 ± 0.05, respectively.
For tilapia, the safe dosages of iodorin-200, caflon BKC 80, formalin, malachite green, and SCI-62 were 0.97 ppm, 0.35 ppm, 16.25 ppm, 0.68 ppm, and 0.48 ppm, respectively. Meanwhile, the safe dosages of the same above tested disinfectants for soft-shell turtle were were 36.6 ppm, 3.30 ppm, 58.25 ppm, 1.49 ppm, and 10.27 ppm, respectively.
From the histopathology studies, the results of the acute toxicities of the disinfectants showed that the second laminar epitheial were hyperplasia and fused. Moreover, we found that there were vacuolization of liver cells and hyaline degeneration epethial cells of renal tube in the tilapia. Howerver, liver cell vaculolization and the epethial cells of renal tube hyaline degeneration were found in soft-shell turtle.
Identifer | oai:union.ndltd.org:TW/088NCHU0540012 |
Date | January 2000 |
Creators | HongYou Chang, 張鴻猷 |
Contributors | WeiShain Wang, 王渭賢 |
Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
Language | zh-TW |
Detected Language | English |
Type | 學位論文 ; thesis |
Format | 103 |
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