碩士 / 國立成功大學 / 化學系 / 88 / In the course of our continuing research for novel biologically activity compounds from natural sources, we were interested to investigate the chemical constituents and bioactivities of the roots bark of Severinia buxifolia Tenore which was collected in Hainan province, and the stems and roots of Cyclea gramillima Diels.
Thirty-one compounds were isolated and characterized from the root bark of S. buxifolia. Including eight new acridone alkaloids, buxifoliadine-A (1), -B (2), -C (3), -D (4), -E (5), -F (6), -G (7), and —H (8); two new tetranortriterpenoids, 7-isovaleroylcycloseverinolide (9) and 7-isovaleroylcycloepiatalantin (10); one new sesquiterpenoid, a-santalane-11,12,13-triol (11), together with twenty known compounds (12~31). On the other hand, thirty-four compounds, contain three new alkaloids, cycleagracine-A (32), —B (33) and —C (34), together with twenty—five known compounds (24, 25, 35~58) were also isolated and characterized from the stems and roots of C. gramillima. Their structures were determined by spectral analysis and chemical transformation methods.
This work and our previous results show that the constitutions of the root bark of S. buxifolia vary according to area. Prenyl and pyrano type acridone alkaloids occur in both Taiwan and Hainan; simple and furano type acridone alkaloids were found only in Hainan. This is the reason why the pharmacological activities of traditional Chinese medicines are related strictly to the collecting area. In this study, we found the methylene carbon of the prenyl groups at C-2 and/or C-4 of the N-unsubstituted acridone alkaloids appear in the region of 21.3~23.9 ppm, which was different from the N-methyl acridone alkaloids in the 13C-NMR spectra. Therefore, it was not used to distinguish the substitution of the prenyl group at C-2 or C-4 in N-unsubstituted acridone alkaloids. Buxifoliadine-B, -D, -H, severifoline, citrusinine-I, -Ⅱwere subjected to evaluate the cytotoxic activity, and showed significant cytotoxicity against KB, Hepa-3B and Colo-205 cell.
| Identifer | oai:union.ndltd.org:TW/088NCKU0065035 |
| Date | January 2000 |
| Creators | Chen Chien-Mao, 陳建茂 |
| Contributors | Wu Tian-Shung, 吳天賞 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 181 |
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