碩士 / 國立屏東科技大學 / 熱帶農業研究所 / 88 / Abstract
The cultivation of shrimp on an industrial scale has rapidly developed in many countries of the tropical region of the world. During the cultural practices, shrimp farmers always applied certain aquatic bactericides for pond treatment. In order to elucidate the effects of the chemicals on the metabolism of the shrimp, the three common used chemicals were chosen to study on the freshwater giant prawn.
The freshwater giant prawn, Macrobrachium rosenbergii, was examined for the variation in osmolality, ionic concentrations, total protein and oxy-hemocyanin contents after being exposed to different concentrations of copper sulfate (CuSO4; at 0, 0.1, 0.2, 0.4 ppm) potassium permangnate (KMnO4, at 0, 0.3, 0.6, 1.0 ppm) and benzalkonium chloride (B.K.C., at 0, 0.3, 0.6, 1.0 ppm). Six prawns were sampled at various time intervals.
Results of the study show that when the prawns were exposed to CuSO4, the osmolality significantly decreased with the increase of concentration of CuSO4. The effect was the greatest at 0.4ppm. Although the osmolality of the test groups showed signs of increase at 96 hr, the test group of higher concentration did not recover to its original level. The ionic contents of sodium and chlorine were synchronized with their respective osmolalities. The osmolality did not have a significant effect on the ionic concentrations of potassium. Contrary to potassium ion, calcium ion significantly increased when exposed to CuSO4 for 24 hr, but the ionic concentrations returned to its original level after 96 hr. An acute change was observed in magnesium ion at 48 hr, but the change was not significant. The contents of the total protein and the oxy-hemocynin increased in 24 hr with the increase of the concentration of CuSO4.
When exposed to potassium permanganate (KMnO4), the osmolality of M. rosenbergii decreased with the increase of the concentration of KMnO4. In the groups with the concentrations of KMnO4 at 0.6 and 1ppm, the osmolality significantly decreased at 24 hr. The ionic concentrations of sodium and chlorine were synchronized with the osmolality. However, there was no significant effect on potassium ion. The calcium ion was highly unstable in KMnO4. Magnesium ion showed a greater change or 24 hr, but it returned to its original concentration at 96 hr. The contents of the total protein and the oxy-hemocynin decreased with the increase of the concentration of KMnO4. When raising the concentration of KMnO4 from 0.3 to 1ppm, the contents of the total protein and the oxy-hemocynin significantly decreased and continued to decrease at 72 hr.
When exposed to B.K.C., the osmolality decreased with the increase of the concentration of B.K.C. The test group at higher concentrations demonstrated more pronounced effects than at lower concentrations. The osmolality significantly decreased in the 0.6ppm group at 48 hr. and in the 1ppm group at 24 hr. The ionic contents of sodium and chlorine appeared to synchronize with their osmolalities. There was no significant effect observed on potassium ion. Calcium ion appeared to be unstable and its contcentration decreased with time. The calcium ion returned to its original concentration at 216 hr. Magnesium ion was not significantly different and appeared to be stable. The contents of the total protein and oxy-hemocynin decreased with the increase of the B.K.C. concentration. The osmolality significantly decreased in the 0.6ppm group at 72 hr and in the 1ppm group at 48 hr.
Based on the results of this study, the osmolality and concentrations of ions, total protein and oxy-hemocynin of M. rosenbergii were directly influenced by the concentrations of copper sulfate, potassium permanganate and B.K.C.
【Keyword】Macrobrachium rosenbergii, Copper sulfate, Potassium permanganate, Benzalkonium chloride, Osmolality, Sodium, Potassium, Chloride, Calcium, Magnesium, Total protein, Oxy-hemocyanin.
| Identifer | oai:union.ndltd.org:TW/088NPUST643017 |
| Date | January 2000 |
| Creators | Chien-Hung Chen, 陳建宏 |
| Contributors | Weng-Chung Chang Ph. D., Winton Cheng Ph. D., 張文重, 鄭文騰 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 85 |
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