碩士 / 國立海洋大學 / 水產養殖學系 / 88 / For the development of the cultivation of red alga Grateloupia filicina, the establishment of seed stock, both filamentous and crust stage in a large quantity is a preliminary step. For this purpose, several environmental factous, e.g. photoperiod, light untensity, temperature were studied for their effects on the regeneration of crusts of G. filicina after wounding treatment. It was found that the shortest light period (4 L:20 D) showed the best effect in maintaining the regeneration filimants at a thorougly filamentous stage. Longer light period (8 L:16 D,12 L:12 D) would favor the formation dark crusts.This dark crusts in photoperiod 12 L:12 D will further develop new shoots. In seaweed aquaculture, the filamentous stage can be kept as seed stock for rope culture while the dark crusts can be used as that for suspension culture.
To further promote the growth of the filaments in a short light period, the addition of plant growth regulators (PGR), polyamine, nitrogen sources and organic carbon sources have been tried in artificial culture medium (ASP12-NTA). The results showed that in single PGR experiment, IAA at concentration of 0.01 mg/L exhibited the best promotion, while in combination experiment with 2,4-D 0.01 mg/L and BA 0.01 mg/L at a ratio of 1:1 the effect was further promoted. In polyamines experiment,putrescine was significantly effective at 0.01 mg/L, when compared to control.In the addition of nitrogen of different sources exhibited various effect on the filament growth. Urea showed no significant effect on the growth of the filaments, that of NaNO3 showed saturation at 50μΜ, while NH4Cl presented an increasing trend of growth at the experimental concentration range (0-200 μΜ). Supplementary organic carbon source also showed different effect in promoting filament growth, with glycerol at 0.3 M exhibited the best effect, glycerol at 0.1 M ranked the second, while the other organic carbon sources was either less effective or not. The addition of glycerol in nature seawater at concentration of 0.1 M exhibited even higher growth promotion on filament growth.
Aside from regeneration of wound thallus filaments seed stock could also be prepared directly from tetraspores by culturing them at non-attachment status and shaking them at suitable interval in a short- light period environment. The morphogenesis pattern could be modified following the change in the regime of photoperiod as described in the previous results.
| Identifer | oai:union.ndltd.org:TW/088NTOU0086018 |
| Date | January 2000 |
| Creators | Huey Chen, 陳卉 |
| Contributors | Chung Sing Chen, 陳忠信 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 90 |
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