碩士 / 國立海洋大學 / 水產養殖學系 / 88 / In the studies, first part of these studies we used a series of methods for enrichment, isolation and identification and also investigate the optimal growing condition of the photobacteria, under different environmental conditions (salinity: 0~35ppt, temperature: 20~40oC, pH: 5.5~9, illuminant: 500~3000lux, C/N ratio: 1~20, sulfide 0.5~2.0mM)
The result showed that on the aspect of organism characteristic: Cell is rod-shaped, 0.5~1.5μm in diameter, motile forms have polar flagella, cells divide by binary fission, and produce capsules and slime, and form chains of cells are straight arrangement. Cultures grown anaerobically in the light are brown, but when cultures are grown in the presence of oxygen, they are red to purple-red. Absorption spectra of living cells show maximum peak on 862~864, 804~806, 588~591, 493~197 and 377~379nm. Photosynthetic pigments are bacteriochlorophyll a. Growth factors required thiamine, according to above data, we can identification this strain was Rhodobacter capsulatus. In addition, the result showed that, the aspect of growth under different environmental conditions, such as the optimum growing condition was temperature 30~35oC, pH 5.5~7.0, salinity 0~5ppt, illuminant 500~2000lux, C/N ratio 5, and sensitivity towards sulfide at 0.5mM.
The second part of these studies was to experiment the effect of application of various combinations of Rb. capsulatus L1 on Cyprinus capio rearing pond wastewater treatment. The combinations of Rb. capsulatus L1 give rise to six treatments (50, 100, 250, 500, 750, 1000 ppm) and one control. Each treatment was under dark aerobic and light anaerobic two different conditions. Regular samplings were done to analyze parameters of water with various water samples at every day during period of five days.
The results showed that six treatments had increased the water pH compared to control, and each treatment reduced the chemical oxygen demand (COD) and nitrite-N in wastewater. In aspect of ammonia-N, under dark aerobic condition, 50ppm and 1000ppm two-treatment had decreased ammonia-N in 72 hour, 96 hour and 120 hour. In addition, under light anaerobic condition, in 1000ppm treatment at first day rapidly decreased ammonia-N and in 50, 100, 250, 500, 750ppm treatments also had decreased ammonia-N compared to control.
The third part of these studies was to experiment the effect of application of the Rb. capsulatus L1 nutrients on Cyprynus capio fry survial rates and percent weight gain. The experimental diets for cyprynus capio fry divide into four groups. A groups: only addition of Rb. capsulatus L1 100ppm. B groups: addition of 1% of Rb. capsulatus L1 with artifact food. C groups: only fed with artificial feed. D groups: fed artificial feed and 50ppm of Rb. capsulatus L1 suspend solution.
After feeding peroid of thirty days, the result showed that, on the aspect of survival rates was A: 32.86%, B: 70.71%, C: 70%, D: 73.5%, and percent weight gain was A: 16.89%, B: 71.93%, C: 60.5%, D: 70.75%, respectively.
| Identifer | oai:union.ndltd.org:TW/088NTOU0086021 |
| Date | January 2000 |
| Creators | chih chung, Lin, 林智中 |
| Contributors | chia lien, Liu, 劉嘉煉 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 95 |
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