碩士 / 國立海洋大學 / 食品科學系 / 88 / Two feeding trials were conducted to determine the adequate level of dietary vitamin B6 (pyridoxine, PN) for juvenile grass shrimp Penaeus monodon and juvenile malabar grouper Epinephelus malabaricus fed diets containing different protein levels.
Experiment 1, purified diets with eight levels of PN (0, 10, 30, 60, 100, 150, 200, 600 mg/kg diet) at either 36 or 44% protein concentration were each fed to three replicated groups of juvenile grass shrimp (mean weight : 0.84±0.05g) for 10 weeks. Results indicated that in the 36% protein group, shrimp fed 60 mg PN/kg had significantly (P<0.05) more weight gain than shrimp fed other PN supplemented diets; feed conversion ratio (FCR) of shrimp fed 60 mg PN/kg was better than the unsupplemented control group. In the 44% protein group, shrimp fed 150 mg PN/kg had higher weight gain than shrimp fed diets supplemented with <100 mg PN/kg and shrimp fed 150 mg PN/kg had better FCR than shrimp fed other PN supplemented diets except 100 mg PN/kg. Protein efficiency ratio (PER) were highest in shrimp fed 60 and 150 mg PN/kg in 36 and 44% protein diets, respectively. The hepatopancreatic glutamate-oxaloacetate transaminase (GOT) activity were highest in shrimp fed 100 mg PN/kg in both 36 and 44% protein groups. Weight gain and hepatopancreatic GOT activity of shrimp analyzed by broken-line regression indicated that the optimum dietary vitamin B6 requirement in juvenile grass shrimp were 31.19-68.49 and 105.10-131.50 mg PN/kg diet in 36% and 44% protein diets, respectively.
Experiment 2, purified diets with eight levels of PN (0, 1, 2, 4, 7, 10, 20, 50 mg/PN kg diet) at either 44 or 50% protein concentration were each fed to three replicated groups of juvenile malabar grouper (mean weight: 3.83±0.07 g) for 8 weeks. Results indicated that fish fed the diet without PN supplementation after 2 wks developed abnormal neurological signs, spiral swimming, hyperirritability and convulsions. In the 44% protein group, fish fed 2 mg/PN kg diet had more weight gain than fish fed 0, 1, 4 and 7 mg/PN kg diet; fish fed 2 mg/PN kg diet had higher PER than fish fed the unsupplemented control diet; fish fed 2 mg/PN kg had higher muscle GOT activity than fish fed 50 mg/PN kg. In the 50 % protein group, fish fed 4 mg/PN kg diet had more weight gain than fish fed other diets; fish fed 4 mg/PN kg had higher PER than fish fed 0, 7, 10 and 20 mg/PN kg diet; fish fed 2 mg/PN kg had higher muscle GOT activity than fish fed 0 and 1 mg/PN kg. Weight gain and muscle GOT activity of fish analyzed by broken-line regression indicated that the optimum dietary vitamin B6 requirement in juvenile malabar grouper were 0.82-1.12 and 1.27-2.89 mg/PN kg diet in 44% and 50% protein diets, respectively.
| Identifer | oai:union.ndltd.org:TW/088NTOU0253005 |
| Date | January 2000 |
| Creators | Ming-Hsun Wu, 吳明勳 |
| Contributors | Shi-Yen Shiau, 蕭錫延 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 108 |
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