碩士 / 國立臺灣大學 / 海洋研究所 / 88 / A search for halophilic, facultatively anaerobic bacteria that ferment carbohydrates with gas production has been made in the present study. The most-probable-number ( MPN ) counting values of these bacteria in the coastal seawater collected from northern Taiwan were estimated to be in the range of 101 to 104 cells/100 ml. Forty-three bacterial strains that ferment carbohydrates with gas production were isolated from the MPN counting tubes. Twelve lost the gas-producing property after subculture among them. All the other isolates were Gram-negative, and motiled by polar flagella. They were able to grow in a mineral medium containing D-glucose, and could ferment carbohydrates with gas production. These isolates required Na+ for growth, too. According to phenotypes, thirty-one strains were divided into five groups. Group 1 contained only one strain, which produced red colonies. The cells were curved rods in shape and oxidase-negative. The strain was able to assimilate mannitol as sole carbon source, and reduced nitrate. Group 2 contained six strains, which produced white and transparent colonies. The cells were curved rods in shape and oxidase-negative. The strains were able to assimilate mannitol as sole carbon source, and reduced nitrate. Group 3 contained three strains, which also produced white and transparent colonies. The cells were curved rods in shape and oxidase-positive. The strains were able to assimilate mannitol as sole carbon source, and couldn’t reduce nitrate. Group 4 contained three strains, which produced white and opaque colonies. The cells were short and straight rods in shape and oxidase-positive. The strains weren’t able to assimilate mannitol as sole carbon source, and reduced nitrate. Group 5 contained eighteen strains, which produced white and transparent colonies. The cells were curved rods in shape and oxidase-positive. The strains were able to assimilate mannitol as sole carbon source, and reduced nitrate. The levels of 16S rDNA sequence similarity compared with any sequences available in the GeneBank database revealed that the representative strains NFG1 and NFG3 of Group 1 and Group 3 could be considered to represent various new species. The representative strain NFG4 of Group 4 was identified as Photobacterium damselae subsp. damselae. The taxonomic status of NFG2 and NFG5 that representative strains of Group 2 and Group 5 couldn’t be determined.
| Identifer | oai:union.ndltd.org:TW/088NTU00279019 |
| Date | January 2000 |
| Creators | Chen, Yi-Wen, 陳怡文 |
| Contributors | Shieh, Wung Yang, 謝文陽 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 68 |
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