碩士 / 國立臺灣大學 / 漁業科學研究所 / 88 / To investigate the peptide-antimicrobial activity relationship of shrimp, bovine lactoferrin (bLF), the basic amino acid-rich region of lactoferricin B, RRWQWRMKKLG (N: 11 mer), yellowfin progy (Acanthopagrus latus) recombinant growth hormone (ypGH) and carp (Cyprinus carpio) recombinant interlukin-1 (cIL-1) were selected. Tiger shrimp (Penaeus monodon) hemocytes were incubated with bLF, N: 11 mer, ypGH and cIL-1, respectively, and quantified the production of superoxide anion (O2) by nitroblue tetrazolium (NBT) staining. All of the peptides could enhance the production of O2 of shrimp hemocytes, which was the first product of phagocytosis. The antimicrobial activities of the peptides were also tested by determining the minimal inhibitory concentration (MIC) of Vibrio vulnificus, Vibrio alginolyticus and Vibrio harveyi, respectively, in the presence of the peptides. In contrast to ypGH and cIL-1, bLF and N: 11 mer were successful to inhibit the growth of the pathogen of tiger shrimp. Finally, the membrane disruptions of tiger shrimp hemocytes that caused by bLF and N: 11 mer, respectively, were determined with trypan blue staining. The survival rate of bLF- and N: 11 mer-treated hemocytes was significant differ with untreated hemocytes after 3- or 1-hour (s) incubation, respectively. These results suggest that bLF and N: 11 mer have the ability to stimulate the immune responses of shrimp hemocytes and to inhibit the growth of pathogen, but the two peptides can decrease the survival rate of shrimp hemocytes.
| Identifer | oai:union.ndltd.org:TW/088NTU00451011 |
| Date | January 2000 |
| Creators | Yu-Chih Chen, 陳玉芝 |
| Contributors | Huai-Jen Tsai, Yen-Ling Song, 蔡懷楨, 宋延齡 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 40 |
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