碩士 / 國立臺灣大學 / 漁業科學研究所 / 88 / Paralytic shellfish poisoning (PSP) toxins which were mainly produced by marine dinoflagellates can be accumulated by bivalves. Because it is strickly harmful to human health, many countries have regulation to protect people from PSP-contaminated shellfish. The PSP toxin standards were finding prepared from culture of toxic dinoflagellates. The goal of this study was the best cultural condition of Alexandrium minutum Halim (AmTK-4) for the highest toxin production and the efficient procedure for purifying individual GTX toxins,
Alexandrium minutum Halim (AmTK-4) isolated from TonKong area were cultured under one variable condition, including temperature (15℃, 20℃, 25℃, 30℃) and salinity (5ppt, 15ppt, 25ppt, 35ppt). Algae cells were collected per 4 days and quantitied toxin contents with certified toxin standards by using HPLC-FD system. The temperatures and salinity for optimal growth of A. mintum were 20-25℃ and 25ppt, respectively. The toxin production rate and toxin content decreased while cultural temperature decreased. In contrast, while salinity increased, toxin content increased. More over, the relative growing stages, the GTX-2, -3 increased during log phase. It was suggested that the GTX-2, -3 were precursors of GTX-4, -1. Under difference cultural conditions, A. mintum showed different growth rate, μ. The relative percentage of GTX-4, -1 were higher while growth rate was higher.
The mass culture of A. mintum under 25ppt and 25℃, were harvested during stationary phase for toxin production. The ethanol extracts of A. mintum further purified by gel filtration chromatography. and ion exchange chromatography. From the mixture of GTX-1, -2, -3, -4 (1.21mg), pure GTX1 (1.77×10-2mg), GTX2 (3.61×10-2 mg), GTX3 (2.06×10-4mg) and GTX4 (0.12 mg) were obtained through two steps of ion exchange chromatography.
| Identifer | oai:union.ndltd.org:TW/088NTU00451014 |
| Date | January 2000 |
| Creators | Jeng Jeng-Shi, 鄭正熙 |
| Contributors | Hung-Non Chou, 周宏農 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 64 |
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