碩士 / 台北醫學院 / 細胞及分子生物研究所 / 88 / Corticotropin-Release Hormone (CRH), a 41 amino acid neuropeptide, is a major regulator of the HPA axis, which release leads to pituitary production of adrenocorticotropic hormone (ACTH) followed by glucocorticoid secretion by the adrenal cortex. CRH has been implicated as an important neuro-hormone participating in seizure generation particularly in early life of both human and animals. It is possible that not only CRH presents as a pro-convulsant in the developing brain, but in seizure activity, it may elicit release of CRH which could further enhance the seizure intensity. To address this issue we measured the plasma CRH concentration in developing rats received kainic acid (KA), a convulsant agent which exerts behavioral and electrophysiological seizure activities by activating at kainate receptor and N-methyl-D-aspartate (NMDA) receptor. We also determine whether the NMDA receptor antagonist-dextromethorphan (DM) could attenuate release of CRH on KA-induced seizure on the release of CRH. These results demonstrated that significantly increase circulating CRH concentration (46.62±30.32 ng/ml, p=0.0303) was observed in P30 rat but not on P7 and P14 rat. Injection of DM could effectively attenuate the KA-induced seizure intensity as well as the CRH level (p=0.0026) on P30 rat. Furthermore, mRNA expression of neuropeptide (CRH and POMC) and cytokines (IL-6, IL-1β, TNF-α, and IFN-γ) from different brain region are analyzed by RT-PCR. In P30 rat cortex, CRH, IL-6 and IL-lβ mRNA expression were significantly increased in KA-induced seizure as compared to control. In addition, CRH mRNA is also significantly increased in P7 rat cortex in KA-induced seizure as compared to control. These data support the CRH and cytokines (IL-lβ and IL-6) may have immunoregulatory role in seizure activity.
| Identifer | oai:union.ndltd.org:TW/088TMC00339011 |
| Date | January 2000 |
| Creators | 陳素蓮 |
| Contributors | Sy-Jye Leu, 呂思潔 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 77 |
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