碩士 / 國立陽明大學 / 微生物暨免疫學研究所 / 88 / In this thesis, we sampled seawater and sediment at the seaside of northern Taiwan and studied several novel microbial strains isolated from the sample. Among all isolates, seven pure strains were picked for further study, and they are designated as strains SR1~SR7 respectively. By using various classification methods, such as morphology, physiological properties, biochemical tests, GC content, and 16S rRNA sequence comparison, strains SR1~SR7 are classified into known categories. Strains SR1~SR7 are all Gram-negative, motile, polar-flagellated, aerobic, rod-shaped eubacteria and can grow in nutrient media containing 0.5%~12% of NaCl. Sodium ion is essential for growth. Strains SR1~SR7 are psychrotrophs and able to grow at 4 oC with optimal growth at 20~25 oC. They are neutrophilic and cannot ferment glucose in the Hugh-Leifson test. All of them are oxidase-positive and catalase-positive. They can produce caseinase and lipase, but not agarase, chitinase, and urease. Some strains of them are able to hydrolyze starch and gelatin. Strains SR1~SR7 cannot reduce nitrate, and their G+C contents of the chromosome DNA are 43%、46%、43~44%、41~42%、46%、48%、47% respectively. The 16S rRNA sequences of our strains are similar to Pseudoalteromonas spp.. Phenotypes of our strains are also close to that of Pseudoalteromonas spp.. Consequently, we propose that strains SR1~SR7 belong to genus Pseudoalteromonas. They are probably new species in the genus Pseudoalteromonas.
| Identifer | oai:union.ndltd.org:TW/088YM000380029 |
| Date | January 2000 |
| Creators | Chin-Chun Liu, 劉芹君 |
| Contributors | Suh-Der Tsen, 程樹德 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 70 |
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