碩士 / 國立陽明大學 / 解剖學研究所 / 88 / Tetramethylpyrazine (TMP), an active principle isolated from the Chinese herb “chuanxiong”, has been shown to protect rat liver, and hippocampus from ischemia injury. The effects of TMP on excitotoxicity induced by kainate is still unknown. Firstly, we induced excitotoxicity in cultured rat hippocampal neurons by exposure to 75 μM-450 μM kainate for 12 h, 24 h, and 48 h, and cell viability was measured using PI and DAPI staining. The result showed the cell damage increased with the time of kainate treatment and reached its peak at 24 h followed by a plateau until 48 h. At the same time, we also investigated the effects of kainate on mitochondrial membrane potential (MMP) and reactive-oxygen species (ROS) production in cultured rat hippocampal neurons. Kainate induced a maximum decrease of 84% in MMP in 1 h, and a maximum increase of 128% in ROS production in 2 h. Furthermore, we also investigated the effects of TMP on kainate-induced ROS production, decrease in MMP, and excitotoxicity. The results demonstrated that pretreatment with TMP inhibited the ROS production, the decrease of MMP, and the excitotoxicity induced by kainate.
| Identifer | oai:union.ndltd.org:TW/088YM000391002 |
| Date | January 2000 |
| Creators | Seling Wu, 吳瑟鈴 |
| Contributors | Yu-Show Fu, Hung-Hai Ku, 傅毓秀, 古宏海 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 75 |
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