碩士 / 中國醫藥學院 / 醫學研究所 / 89 / Heat shock proteins (HSPs) are induced by a variety of insults in the nervous system. We investigated the expression of HSP70, HSP27 and the apoptosis-associated Bax protein in hippocampal subfields of lobectomy specimens from temporal lobe epilepsy (TLE) patients. The pathologic findings showed severe hippocampal neuron losses and gliosis in surgical specimens. The mean percentage of apoptotic cells detected by TUNEL method in the hippocampus was 9.02% and that in the temporal lobe was 2.24%. It was statistically higher of apoptotic cells in the hippocampus compared with the temporal lobe in TLE (P<0.005). The increased expression of apoptosis-promoting Bax protein was observed in the hippocampus in all cases of TLE, whereas, there were weak and few signals in the temporal lobe in TLE and non-epileptic control autopsied tissue. Increased immunoreactivities for HSP70 and HSP27 were observed in glial cells in the hippocampus in TLE. HSP70 was detectable in 7.54% of glial cells in the hippocampus and HSP27 was 3.29%. The percentages of HSP70 and HSP27-positive cells were statistically higher in the hippocampus than in the temporal lobe (P<0.005 and P<0.05). We interpret these interesting findings as evidence of a possible apoptotic mechanism, which lead to hippocampal cell death in TLE. The expressions of HSP70 and HSP27 in glial cells in the hippocampus suggest that they might play some role in remolding the injured region of the CNS.
| Identifer | oai:union.ndltd.org:TW/089CMCH0534004 |
| Date | January 2001 |
| Creators | I-Hua Chou, 周逸華 |
| Contributors | Chao-Tien Hsu, 許朝添 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 66 |
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