碩士 / 逢甲大學 / 化學工程學系 / 89 / Biocatalysts are often immobilized when they are applied in practical operations. However, conventional immobilization methods, such as matrix entrapment, usually cause mass transfer restriction that leads to a decrease in the efficiency of the biocatalysts. In this study, an azo-dye-decolorizing bacterium Pseudomonas luteola was used as the biocatalyst to remove the color of azo dye Reactive Red 22. A hollow-fiber microfiltration membrane was utilized to retain the biocatalyst inside the reactor for continuous decolorization of the azo dye. The membrane reactor causes no mass transfer limitations, and may also raise the cell concentration within the reactor; thus has the potential to enhance the operation efficiency.
This study utilized the hollow-fiber membrane reactor for continuous decolorization of wastewater. The optimal operation strategy was determined by investigate the effects of operation parameters (e.g., temperature, hydraulic retention time (HRT), dye loading rate, medium loading rate, and biomass concentration, etc.) on decolorization efficiency. The results show that the best decolorization rate occurred at a temperature of 40oC. The overall decolorization efficiency increased when biomass concentration was increased. The optimal HRT was 13 h. The decolorization rate increased rapidly with an increase in dye loading rate. The extracellular metabolites of a dye-decolorizing bacterium Escherichia coli NO3 appeared to enhance the decolorization ability of P. luteola, and the maximal decolorization was elevated to 125 mg dye/g cell/h.
| Identifer | oai:union.ndltd.org:TW/089FCU00063002 |
| Date | January 2001 |
| Creators | Chao-Shuan Chang, 張肇栓 |
| Contributors | Ping-Jei Lin, 林屏杰 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 85 |
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