碩士 / 國立屏東科技大學 / 獸醫學系 / 89 / More than 100 difference herpesviruses have been described in higher and lower vertebrates as well as in invertebrates. However, there is no report to describe the herpesvirus infection in soft-shelled turtles. In this study, clinical signs observed in diseased cases were lethargy, anorexia. Histopathologically, eosinophilic inclusion bodies were detected in spleen, liver, intestine, lung, stomach, pancreas, kidney, urinary bladder, skin, and eye. The electron microscopic examination of the cells containing inclusion bodies showed virus-like particles measuring 65~75 nm and 250~260 nm for nucleocapsids and mature enveloped particles present in the cytoplasm. Several mammals, fish, turtle cell lines, and soft-shelled turtle embryos cell were used to isolate the virus. But virus was unable to be isolated in this study. The viral DNA sequence of 246 bp was amplified directly from tissues of related diseased soft-shelled turtle cases by polymerase chain reaction. The amplified fragments were shown to be specific for the target genes by in situ hybridization. Sequence of amplified product was analyzed and indicated that this virus was most closely related to members of the herpesvirus family. Based upon the morphology（size and location）and sequence analysis, we suggest that this virus is the soft-shelled turtle herpes-like virus（STHLV）.
| Identifer | oai:union.ndltd.org:TW/089NPUST541012 |
| Date | January 2001 |
| Creators | woo cheng-hsing, 吳承興 |
| Contributors | tsai shinn-shyong, 蔡信雄 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 65 |
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