碩士 / 國立清華大學 / 化學工程學系 / 89 / In this study, we mainly discussed that using tide-type bioreactor to culture CHOβ-gal 37 cell lines which could expressβ-gala- ctosidase and probe into that serum concentration and additives make what effect upon the production.
In the experiment of stationary culture, we have cultured plenty of CHOβ-gal 37 cells and preserved them successfully. The effect of serum concentration on cell growth, CHO cells cultured in IMDM media with 5% FBS grew faster and produce moreβ-gal.
To probe into the effect of additives on cell growth, adding 2.4g/L peptone in serum-low media could enhance the growth of CHO cells, but could not raise the production of β-gal. The growth rate of CHO cells treated with 1% DMSO decreased gradually with increase in specific activity. Therefore, the best scheme in stationary culture is that culturing CHO cells in serum-low media without DMSO, and then replacing media with DMSO at fourth day.
In the aspect of bioreactor culturing, we have successfully cultivated CHO cells in tide-type bioreactor. When cells grew below the liquid surface in bioreactor, the number of cells could arrive above 2.5 x 106 cells. Furthermore, we observed that vertical moving of air-water interface would result negative effect on cell growth in tide range.
Tide-type bioreactor could be considered an ideal bioreactor because it is easily operated and have a low shear force, bubble-free condition for cell culture. In future prospection, we would probe into the decided mechanism of DMSO and find out better substitutes of serum. In tide-type bioreactor, we want to improve tide-type bioreactor on culturing cells efficiently and study the reason of inhibition of cell growth in tide range or to prevent this phenomenon.
| Identifer | oai:union.ndltd.org:TW/089NTHU0063013 |
| Date | January 2001 |
| Creators | Kai-Li Lin, 林凱理 |
| Contributors | I-Ming Chu, 朱一民 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 0 |
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