碩士 / 國立海洋大學 / 食品科學系 / 89 / A series of two experiments were conducted to study the influence of dietary methionine on the choline and betaine requirements for juvenile grass prawn, Penaeus monodon.
ExperimentⅠ, basal diet containing 0.89% of methionine supplemented with 7 levels (0, 200, 500, 1,000, 2,000, 4,000, 6,000 mg/kg diet) of choline were fed to P. monodon (mean body weight 0.440.00 g) for 8 weeks. Each diet was fed to triplicate group of shrimp. Results indicated that shrimp fed diet supplemented with 1,000 mg choline/kg diet had significantly (P<0.05) higher weight gain (WG) and protein efficiency ratio (PER) than shrimp fed the unsupplemented control diet. Hepatosomatic index (HSI) was higher in shrimp fed 1,000 and 2,000 mg choline/kg diets than the shrimp fed the control diet. Hemelymphic triglyceride (TG) and phospholipid (PL) concentrations were lower in shrimp fed 2,000 mg choline/kg diet than shrimp fed other diets. Analysis of WG, HSI and PER of the shrimp by polynomial regression indicate that the adequate dietary choline requirement in grass prawn is approximately 1,680~1,800 mg choline/kg diet.
ExperimentⅡ, diets with 2 dietary choline levels (0 and 1,680 mg/kg diet), each supplemented with 8 betaine levels (0, 300, 600, 900, 1,200, 2,000, 4,000, 6,000 mg/kg diet) were prepared. There were total of 16 diets, each diet was fed to triplicate groups of shrimp (mean body weight 0.210.05 g) for 8 weeks. In the 1,680 mg choline/kg supplementation group, shrimp fed 2,000 mg betaine/kg and 6,000 mg betaine/kg diets had higher WG, FE, PER and higher whole body and hepatopancreatic betaine concentrations, respectively than shrimp fed the betaine-free control group. In the 0 mg choline/kg supplementation group, WG, FE and PER were similar in all the dietary groups regardless of the betaine supplementation level; shrimp fed 4,000 mg and 1,200 mg betaine/kg diets had higher whole body and hepatopancreatic betaine concentration than shrimp fed the betaine-free control diet. Analysis of WG and body betaine concentration of the shrimp by broken line regression indicate that the adequate dietary betaine supplementation in grass shrimp is approximately 1,500 mg/kg diet when the diet contains adequate amount of methionine (0.89%) and choline (1,680 mg/kg).
| Identifer | oai:union.ndltd.org:TW/089NTOU0253039 |
| Date | January 2001 |
| Creators | Wang Yi Ting, 王意婷 |
| Contributors | Shiau Shi Yan, 蕭錫延 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 87 |
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