碩士 / 國立海洋大學 / 食品科學系 / 89 / Abstract
To investigate the dietary selenium (Se) requirement and its interaction with vitamin E in juvenile tilapia, Oreochromis niloticus × O. aureus, diets with 3 levels of vitamin E (HE: 280, ME: 70 and LE: 17.5 mg/kg), each supplemented with 8 selenium levels (Na2SeO3; 0, 0.05, 0.1, 0.2, 0.3, 0.5, 1.0 and 5.0 mg/kg) were prepared. There were total of 24 diets each was fed to triplicate groups of tilapia (mean initial weight: 1.10±0.03 g) in a close-recirculated rearing system for 8 weeks. Weight gain, feed conversion ratio (FCR), survival, protein efficiency ratio (PER) and hepatosomatic index (HSI) of the fish were similar (P>0.05) in all the dietary groups. In the HE group, highest (P<0.05) hepatic Se concentration was in fish fed diets supplemented with 5.0 mg Se/kg, followed by fish fed diets with 0.5, 1.0 mg/kg, and lowest in fish fed diets with <0.3 mg Se/kg. Hepatic vitamin E concentration was high in fish fed diets supplemented with 0, 0.05 and 0.3 mg Se/kg, followed by fish fed the diets with 0.1, 0.2 and 0.5 mg Se/kg, and lowest in fish fed the diets with 1.0 and 5.0 mg Se/kg. Highest hepatic Se-GPX activity was observed in fish fed diets with 0.2, 0.3 mg Se/kg, then in fish fed <0.05 mg Se/kg diet, and lowest in fish fed diets with >0.5 mg Se/kg. In the ME group, hepatic Se concentration was highest in fish fed diet with 5.0 mg Se/kg, followed by fish fed diets with 0.3-1.0 mg Se/kg, then fish fed diets with 0.1, 0.2 mg Se/kg, and lowest in fish fed diet with 0 and 0.05 mg Se/kg. Hepatic vitamin E concentration was highest in fish fed diets with >0.5 mg Se/kg, followed by fish fed 0.05-0.3 mg Se/kg diets, and lowest in fish fed the unsupplemented control diet. Se-GPX activity was high in fish fed diet with 5.0 mg Se/kg, followed by fish fed diets with 0.1-1.0 mg Se/kg, and lowest in fish fed diets with <0.05 mg Se/kg. In the LE group, hepatic Se concentration were highest in fish fed diets with >1.0 mg Se/kg, followed by fish fed diets with 0.2-0.5 mg Se/kg, then fish fed diets with 0.05-0.1 mg Se/kg diet, and lowest in fish fed the unsupplemented diet. Hepatic vitamin E concentration was highest in fish fed diet with 5.0 mg Se/kg, followed by fish fed 0.3-1.0 mg Se/kg diets, then 0.05-0.2 mg Se/kg diets, and lowest in fish fed the unsupplemented diet. Se-GPX activity was highest in fish fed diets with >0.5 mg Se/kg, followed by fish fed 0.2-0.3 mg Se/kg diets, and lowest in fish fed <0.1 mg Se/kg diets. Hepatic Se-GPX activity was higher in HE group than that in ME and LE groups when Se supplementation level was <1.0 mg/kg diet. Weight gain and hepatic Se-GPX activity were analyzed by broken-line method indicates that the optimum selenium requirements in growing tilapia were 0.28 and 0.25 mg/kg, respectively.
| Identifer | oai:union.ndltd.org:TW/089NTOU0253041 |
| Date | January 2001 |
| Creators | 林郁敏 |
| Contributors | 蕭錫延講座 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 0 |
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