碩士 / 國立陽明大學 / 解剖暨細胞生物學研究所 / 89 / The main aim of this study is to explore the protection mechanism of glial-derived neurotrophic factor (GDNF) during excitotoxicity in hippocampus by kainic acid (KA). After microinjected with KA for 15 minutes, the excitotoxicity was induced in the hippocampus of rat. The protection effect of GDNF in the hippocampus was evaluated by administrating the GDNF at 14 minutes after KA injection. First of all, the resulting hydroxyl free radical and dopamine were quantitated by microdialysis of hippocampus and the results show: GDNF is able to effectively suppress the production of KA-induced hydroxyl free radical and prevent the KA-induced dopamine released from the mesodopaminergic nerve fiber. Meanwhile, the histological observation has also indicated that GDNF could decrease the damage level of pyramidal neurons in the CA3 and CA4 areas of hippocampus. In the other hand, the activity of superoxide dismutase (SOD) in the hippocampus has been significantly elevated at 30 minutes and 7 days after the KA induction while the activity of glutathione peroxidase (GPx) was not significantly increased until 7 days. With the GDNF treatment, similarly, activities of SOD and GPx in the hippocampus were significantly elevated at 7 days after the KA induction. The in vitro study further confirmed that GDNF is a quick and potent free radicals scavenger with a dose-dependent capability and, therefore, able to protect the hippocampal neurons and reduce the KA-induced excitotoxicity.
| Identifer | oai:union.ndltd.org:TW/089YM000391008 |
| Date | January 2001 |
| Creators | Jiun-Wen Guo, 郭俊文 |
| Contributors | Yu-Show Fu, Henrich Cheng, 傅毓秀, 鄭宏志 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 94 |
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