碩士 / 中山醫學大學 / 生物化學研究所 / 90 / The tumor suppressor p53 is the most frequently mutated protein in human cancers. Recently, homologues of p53, such as p73 and p63, were isolated in human and mice. Instead of acting as tumor suppressors, these proteins were shown to play important roles in animal development. In this study, we cloned the p73 cDNA from zebrafish, using ovary RNA and methods of reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). Sequencing of four overlapping clones revealed a consensus sequence that contains a 1923-bp open reading frame (ORF) and flanking regions of 37-bp and 438-bp at the 5’- and 3’-ends, respectively. This ORF encodes a 640-amino-acid polypeptide which shares 95%, 71%, 71%, 70% and 32% homologies to p73 of Barbul barbus, Mus musculus, Homo sapiens, Cercopithecus aethiops and Mya arenaria, respectively. RT-PCR analysis revealed that zebrafish p73 was only expressed in restricted tissues such as skin, fin, brain, ovary and testis, in contrast to the more ubiquitous expression of zebrafish p53 and p63. During embryonic development, expression of p73 was detected at 3 hours post fertilization (hpf), and thereafter through 120 hpf. By whole mount in situ hybridization and immunohistochemical staining, we also found that the p73 was expressed in the olfactory bulb—like region and in specific cell types of the reproductive organs. The differences in spatial and temporal expression patterns among p73, p63 and p53 of zebrafish suggest that there are specific functional roles for the three genes.
| Identifer | oai:union.ndltd.org:TW/090CSMU0107005 |
| Date | January 2002 |
| Creators | Hung-Nan Tung, 董鴻楠 |
| Contributors | Chen L Y, Pan Hui Chi, 陳凌雲, 潘惠錦 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 73 |
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