碩士 / 國立中興大學 / 農藝學系 / 90 / Studies on genetic variation of Houttuynia cordata in Taiwan.
Summary
This studies on genetics variation of populations and cultured lines of Houttuynia cordata Thunb. in different zones of Taiwan was preserve of germplasms and development and application of products for reference. From July to December in 2000, wild populations and cultured lines were collected in each zone of Taiwan, and then preceded a gamogenesis in the greenhouse of National Chung Hsing University. 150 days later, plant characters of each populations and lines were investigated. Moreover, applying RAPD to analysis polymorphisms of populations and lines can realize variation of genomes. The results were as follows:
1.Of the populations of H. cordata Thunb., in different zones of Taiwan, the means of plant characters, relationship among characters, and statistics of Hotelling’s T2 all showed the significant difference of ecology among populations. 3 clusters were be classified in ten populations according to analysis of principal component analysis and cluster analysis: The cluster Ⅰ including Chilung, Fuhsing, Shengan, Renai, Lukang, Meinung and Hengchun, the cluster Ⅱ including Yangmin and Taitung, and the cluster Ⅲ including Suao.
2.Utilizing 40 primers proceed to RAPD for 10 populations of H. cordata Thunb. of Taiwan, 14 primers could showed polymorphisms, and all 101 bands appear, and 72 bands would be regarded as the markers. The similarity coefficient of populations, from 0.341 to 0.988, showed populations have greater genetic variation. 3 clusters were be classified according to cluster analysis : the cluster Ⅰ including Chilung, Yangmin, Meinung, Shengan, and Renai, the cluster Ⅱ including Fuhsing, Taitung and Hengchun, and cluster Ⅲ including Suao.
3.Using plant characters of 24 H. cordata Thunb. cultured lines to proceed with ANOVA found significant difference among lines.
4.Utilizing 14 primers proceed with RAPD for 24 cultured lines of H. cordata Thunb. of Taiwan, only 8 primers could showed polymorphisms, and all 23 bands appear, and 18 bands would be regarded as the markers. The similarity coefficient of lines, from 0.281 to 1.000, also showed populations have great genetic variation. 2 clusters were be classified according to cluster analysis: the cluster Ⅰ, 21 lines, inclusive of Taitung 1, Yunlin, Luodong, Hualian, Chishang, Changbin 1, Donghe, Taimali, Taitung 2, Changbin 2, Chenggong, Yuanli 1, Kouhu, Caotun, Xinshe, Fengyuan, Cuoxing, Taipei, Tamsui, Longtan, Fangyuan, and the cluster Ⅱ , 3 lines, inclusive of Pitou, Yuanli 2, Yongjing.
| Identifer | oai:union.ndltd.org:TW/090NCHU0417034 |
| Date | January 2002 |
| Creators | Wei-Chung Chang, 張韋琮 |
| Contributors | Tzer-Kaun Hu, Ph. D., 胡澤寬 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 89 |
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