碩士 / 國立成功大學 / 化學系碩博士班 / 90 / In this paper, a stereoselective esterase cloned from Pseudomonas putida was expressed in E.coli and used for optical resolution of DL-β-acetylthioisobutyamide (ATIA) to get D-β-acetylthioisobutyric acid (DAT). DAT is a precursor of an antihypertensive drug ---Captopril. During hydrolysis of DL-β-acetylthioisobutyamide by the esterase, the pH of the bulk solution was not changed. The activity of the enzyme can be maintained and reused. The advantages of using immobilized cell in the packed-bed reactor is to avoid the multiple steps of enzyme purification and immobilization. In addition, the purification of D-β-acetylthioisobutyric acid from the reaction mixture is also facilitated in the packed-bed reactor in.
| Identifer | oai:union.ndltd.org:TW/090NCKU5065035 |
| Date | January 2002 |
| Creators | Phil Sun, 孫鍾仁 |
| Contributors | Shyh-Yu Shaw, 蕭世裕 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 34 |
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