碩士 / 國立成功大學 / 生物學系碩博士班 / 90 / Abstract
Sequence variation of the atpB-rbcL noncoding spacer region of the chloroplast DNA and RAPD fingerprints were used to reconstruct the phylogeography of Hygrophila pogonocalyx. According to the criteria of the International Union for Conservation of Nature and Nature Resources, H. pogonocalyx is on the Red List Category due to its endangered status. Entomophilous plants of H. pogonocalyx are mostly pollinated by honeybees. Gene flow between populations is expected to be constrained by the migratory capacity of the pollinators. Hierarchical AMOVA analyses of RAPD fingerprints data, which used 50 random primers, indicated significant differentiation between geographical regions (Φct = 0.934; P = 0.048), among populations (Φst = 0.945; P < 0.001), and among populations within region (Φsc = 0.169; P < 0.001). A total of 849 bp of the cpDNA atpB-rbcL spacer was sequenced from 8 populations of H. pogonocalyx. Two geographically based groups, west and east area, were identified by a neighbor-joining tree of cpDNA. Both data revealed that the distribution of genetic variation followed an "isolation by distance" model (in cpDNA, R=0.745). Phylogeographic analysis based on the cpDNA network suggests that the present-day west and east areas of H. pogonocalyx is a result of past fragmentation. The differentiation between geographic regions may be ascribed to a long isolation since the formation of the Central Mountain Range five million years ago.
| Identifer | oai:union.ndltd.org:TW/090NCKU5112005 |
| Date | January 2002 |
| Creators | Wei-Kuang Wang, 王唯匡 |
| Contributors | Tzen-Yuh Chiang, 蔣鎮宇 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 83 |
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