碩士 / 國立屏東科技大學 / 熱帶農業研究所 / 90 / Using a light microscope to study Spirulina sp. or Chlorella sp., which were obtained from the Marine Products Research Institute at Tungkang, Pingting County, only its external morphology, as spiral or round shape, could be observed. However, when supplemented by a transmission electron microscope, its internal structures could be determined, showing septa, thylacoid and a not-well-defined nucleus in Spirulina sp. and cilia, pyrenoid and thylacoid in Chlorella sp. When comparing rDNA of Spirulina sp. and Chlorella sp. with those deposited in GenBank, the results showed that there was 100% similarity between Spirulina sp. and Arthrospira maxima (AJ292323) and about 71-76% similarity between Chlorella sp. and Nannochloris sp.
The study on the ability of the free radical DPPH in eliminating antioxidation showed that, when comparing A. maxima and Chlorella sp. with the standards VitE and BHA under the same concentration of 0.4mg/ml, the antioxidative activities of these two algae were about half of that of VitE and BHA. The antioxidative activities were arranged on the basis of their intensity, in a descending order, VitE>BHA>Chlorella sp. >A. maxima. When the concentration was increased to 1.2 mg/ml, the antioxidative activities of A. maxima and Chlorella sp. increased to about 90%, which was close to that of VitE and BHA. That intensity was at its peak at this concentration and remained so in a stable condition. After purifying through the DEAE anion exchange column, the activities of the superoxide dismutase (SOD) of A. maxima. were 875U/mg, which was 18.3 times more than that of its initial extracts. The same activities of Chlorella sp. were 960U/mg, about 17.9 times of its initial extracts. This result showed that the SOD’s of these two algae were three times higher than that of Ganoderma lucidum and mushroom, demonstrating that there were high SOD activities in A. maxima and Chlorella sp. A. maxima and Chlorella sp., therefore, have a high potential for developing into pharmaceutical or health-related products, emphasizing as having specialized antioxidative characteristics.
| Identifer | oai:union.ndltd.org:TW/090NPUST643029 |
| Date | January 2002 |
| Creators | Shu-Chu Lin, 林淑珠 |
| Contributors | Yeh Wen Ji, 葉文吉 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 90 |
Page generated in 0.7267 seconds