碩士 / 國立中山大學 / 海洋資源學系研究所 / 90 / In order to search for bioactive substances from marine organisms,the Taiwanese soft coral Clavularia inflata,collected along the coast of Green Island,Taiwan,was studied because of its significant cytotoxicity against NUGC-3 and HONE-1 cancer cell lines in EtOAc extract. Bioassay-guided fractionation resulted in the isolation of five diterpenoids,including four known compounds, ( 1R*, 12R* ) - dolabella- 4 (16), 7, 10- triene- 3, 13- dione (1),stolonidiol monoacetate (2), stolonidiol (3),clavutriolin (5),and one new compound,2- acetoxy- stolonidiol (4).
Structures of these compounds were determined on the basis of spectroscopic evidence and chemical derivatization. The application of NMR techniques included 1D- and 2D- NMR,such as COSY, HMQC,HMBC and NOESY,etc. Compounds 1-5 are bicyclic dolabellane skeleton diterpenes. Compound 1 has two ketone groups at C-3 and C-13 positions,an exocyclic methylene at C-4 and two double bonds at C-7 and C-10 positions. Compounds 2-4 possess two epoxides at C-7,C-8,C-10,and C-11. Among them,compound 2 has an acetoxyl group at C-17,and a hydroxyl group at C-18. Compound 3 has two hydroxyl groups at C-17 and C-18 positions. Compound 4 has two hydroxyl groups at C-17 and C-18,and an acetoxyl group at C-2. Compound 5 contains three hydroxyl groups at C-8,C-17,and C-18,and a chlorine atom at C-7, and a epoxy moiety at C-10 and C-11 positions.
The ctytotoxicity of these compounds and their derivatives against KB（human oral epidermoid carcinoma）,Hepa（human liver carcinoma）and Hela（human cervix epitheloid carcinoma）cancer cell lines were also measured. Compounds 1-3 and 5 exhibited significant cytotoxicity against KB cancer cell lines with ED50 values of 0.87,3.07, 1.35 and 0.36 mg/ml,respectively,meanwhile, compound 4 exhibited cytotoxicity against Hepa cancer cells with ED50 value of 1.20 mg/ml. However,the acetylated derivatives 6 and 7 were inactive against these cell lines.
| Identifer | oai:union.ndltd.org:TW/090NSYS5277009 |
| Date | January 2002 |
| Creators | Yu-Ling Pan, 潘玉玲 |
| Contributors | Ya-Ching Shen, 沈雅敬 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 123 |
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