碩士 / 國立海洋大學 / 水產養殖學系 / 90 / Astaxanthin (AS) has been found for tiger prawn Penaeus monodon to increase its resistance to stress and concomitantly the survival. It has been also demonstrated vitamin A (VA) a requirement for P. monodon. AS and VA may involve each other in the roles to play in nutrition. This study therefore aimed to evaluate the effects of combinations of AS and VA in diet on growth and survival of P. monodon and AS, VA, and activities of antioxidase in its body.
Seven experimental diets were used. Serving as a blank, Diet B contained no AS and VA. Diets for six treatments their concentration for AS and VA together was 100 mg/kg diet. D0 contained 0 mg AS but 100 mg (or 275000IU equivalent) VA/kg. D100 had 100 mg AS/kg but no VA. D20, D40, D60, and D80 had AS 20 mg, 40 mg, 60 mg, and 80 mg and also VA 80 mg, 60 mg, 40 mg, and 20 mg/kg, respectively. Each diet trial had 4 replicates.
After 12-week rearing, D20 prawn had best survival and growth. Body astaxanthin was dominated by esterified astaxanthin, of which diester astaxanthin (DA) was more abundant than monoester astaxanthin (MA). Free astaxanthin (FA) was in insignificant amount. Body FA decreased with rearing duration and increasing astaxanthin fraction (AF) in diet. MA decreased with decreasing AF. Total astaxanthin (TA) increased linearly with AF and rearing duration accelerated the increasing trend. Complicate interactions made the change in body VA unparallel along AF and rearing duration, appearing as a right-skewed saddle. Diet VA was transformed to part of body FA, but not to MA or DA. Diet FA could be transformed completely to body VA. When diet AF increased, in a steady fashion, the activity of total antioxidant status (TAS) and glutathione reductase increased and superoxide dismutase (SOD), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) decreased. TA, MA, and DA had significant correlations with all 5 enzymatic activities. Body FA was correlated with AST and ALT. Body VA was not correlated with any of the 5 enzymatic activities. Diet AS but not VA enhanced antioxidant capability and improved hepatopancreas function in P. monodon.
| Identifer | oai:union.ndltd.org:TW/090NTOU0086027 |
| Date | January 2002 |
| Creators | Chih-Chung Huang, 黃志忠 |
| Contributors | Yew-Hu Chien, 陳 瑤 湖 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 64 |
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