碩士 / 國立海洋大學 / 水產養殖學系 / 90 / Abstract
This paper deals with the effects of intrinsic factors like gender, size, molt stages and feeding rate, and the effect of extrinsic factors like ammonia, nitrite and copper sulfate on the immune response of Litopenaeus vannamei. Immune tools used are total haemocyte count (THC), hyaline cell, granular cell, phenoloxidase, respiratory burst and superoxide dismutase (SOD). L. vannamei challenged with Vibrio alginolyticus were placed in water containg different concentrations of ammonia, nitrite and copper sulfate, and the mortality of shrimp was also studied.
No significant differences in THC, hyaline cell, granular cell, phenoloxidase and respiratory burst were observed between male and female shrimp and among shrimp weightd from 3.68 to 30.38 g. Among molt stages of A, B, C, D0/D1 and D2/D3, the THC, hyaline cell, granular cell, phenoloxidase and respiratory burst in C stage are higher than those in A stage. The shrimp with no feeding or feeding rate at 0.1﹪showed significantly lower THC and phenoloxidase activity after 7 days.
The mortality of L. vannamei challenged with V. alginolyticus at 1 x 106 cfu shrimp-1 and exposed to 5.5 mg l-1 ammonia-N was significantly higher than that of shrimp challenged with V. alginolyticus at same dose and exposed in the control solution. L. vannamei following 2 days exposure to 11.17 and 22.32 mg l-1 ammonia-N decreased its SOD, but increased its respiratory burst, and the shrimp following 7 days exposure to 11.17 and 22.32 mg l-1 ammonia-N decreased its phenoloxidase activity. However, no significant differences in THC, hyaline cells and granular cells were observed among the shrimp exposed to 0, 1.07, 5.40, 11.17 and 22.32 mg l-1 ammonia-N.
The mortality of L. vannamei challenged with V. alginolyticus at 1 x 106 cfu shrimp-1 and exposed to 5.5 mg l-1 nitrite-N was significantly higher than that of shrimp challenged with V. alginolyticus at same dose and exposed in the control solution. L. vannamei following 2 days exposure to 4.94 mg l-1 nitrite-N decreased its THC and phenoloxidase activity, and the shrimp following 7 days exposure to 4.94 mg l-1 nitrite-N increased its respiratory burst.
The mortality of L. vannamei challenged with V. alginolyticus at 3 x 105 cfu shrimp-1 and exposed to 5 mg l-1 Cu2+ was significantly higher than that of shrimp challenged with V. alginolyticus at same dose and exposed in the control solution. L. vannamei following 24 h exposure to 5 mg l-1 Cu2+ decreased its THC and phenoloxidase activity, but increased its respiratory burst.
The present study documented that no significant difference in immune ability was observed between male and female L. vannamei, and among the shrimp weighed from 3.68 g to 30.38 g. L. vannamei with no feeding or the shrimp at feeding rate of 0.1 % decreased its immune ability. L. vannamei challenged with V. alginolyticus decreased its resistance when exposed to 5.5 mg l-1 ammonia-N, 5.5 mg l-1 nitrite or 5 mg l-1 Cu2+ .It is concluded that L. vannamei exposed to 11.17 mg l-1 ammonia-N, 4.94 mg l-1 nitrite-N or 5 mg l-1 Cu2+ decreased its immune ability by reducing its phenoloxidase activity. Management of water quality and maintaining better immune ability of cultured animals are suggested during shrimp farming.
Keyword: Litopenaeus vannamei, gender, size, molt cycle, feeding rate, ammonia, nitrite, copper sulfate, total haemocyte count, hyaline cell, granular cell, phenoloxidase activity, respiratory burst, superoxide dismutase, Vibrio alginolyticus.
| Identifer | oai:union.ndltd.org:TW/090NTOU0086029 |
| Date | January 2002 |
| Creators | Tseng I Ting, 曾怡婷 |
| Contributors | Chen Hung Min, 陳鴻鳴 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 83 |
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