碩士 / 國立海洋大學 / 食品科學系 / 90 / The study was aimed at quantifying the optimal vitamin B1 (B1) requirements of juvenile hybrid tilapia (Oreochromis niloticus O. aureus) and grouper (Epinephilus malabaricus). In Experiment I, purified diets with 8 levels (0, 0.5, 1.0, 2.0, 3.0, 4.0, 8.0, 16.0 mg/kg diet) of supplemental vitamin B1 were each fed to triplicate groups of tilapia (mean initial body weight 0.63 0.01 g) for 8 weeks. Results indicated that fish fed the unsupplemented vitamin B1 control diet had lower (P<0.05) weight gain (WG), feed efficiency (FE), protein efficiency ratio (PER) and vitamin B1 concentration in tissues than fish fed diets supplemented with B1. Hepatic and blood transketolase (TK) activity increased, while thiamin pyrophosphate (TPP) effect decreased in fish as the dietary B1 supplementation level increased. Analysis of the WG, blood B1 concentration and TK activity of the fish by broken-line regression indicate that the optimal dietary vitamin B1 requirement for tilapia is approximately 1.4-2.1 mg/kg diet. In Experiment II, purified diets with 8 levels (0, 0.5, 1.0, 2.0, 3.0, 6.0, 10.0, 20.0 mg/kg diet) of supplemental B1 were each fed to triplicate groups of grouper (mean initial body weight 4.09 0.08 g) for 8 weeks. Results indicated that fish fed the B1 unsupplemented control diet had lower WG, FE, PER and tissue vitamin B1 concentration than fish fed diets supplemented with B1. Analysis of the WG, blood B1 concentration and TK activity of the fish by broken-line regression indicate that the optimal dietary vitamin B1 requirement for grouper is approximately 0.7-1.0 mg/kg diet.
| Identifer | oai:union.ndltd.org:TW/090NTOU0253005 |
| Date | January 2002 |
| Creators | Hsiao-Mei, Lin, 林筱玫 |
| Contributors | Shi-Yen, Shiau, 蕭錫延 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 91 |
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