碩士 / 國立海洋大學 / 食品科學系 / 90 / Mackerel scad (Decapterus macarellus) was caught in Taiwan. After cooking and homogenizing, the muscle protein was hydrolyzed by commercial proteases (Prozyme 6, Protease N, and Flavorzyme). Enzymatic hydrolysis was done at 50°C for 2 and 5 hours, respectively. The effect of hydrolysate on inhibitory activity against angiotensin I converting enzyme (ACE) and antihypertensive effect were investigated. The molecular weight of different hydrolysate was all below 1,500 Da. The maximal soluble protein content was obtained when mackerel scad muscle protein was hydrolyzed by Prozyme 6 for 2 hours. The maximal free amino acids and peptide content was obtained when mackerel scad muscle protein was hydrolyzed by Prozyme 6 for 5 hours. Flavorzyme hydrolysis resulted in hydrolysate of little bitterness.
The IC50 value of ACE for the hot water extract was 7.14 mg/ml (nonpreincubation type) and 8.73 mg/ml (preincubation type), respectively. The ACE inhibitory activity of all hydrolysate was increased by enzymatic hydrolysis. The hydrolysate using Prozyme 6 hydrolysis for 5 hours showed the highest inhibitory activity, and the IC50 value was 1.02 mg/ml (nonpreincubation type) and 3.17 mg/ml (preincubation type), respectively. The hydrolysate was separated into three fractions by gel filtration eluted with deionized water. The fraction I (M.W. = 1,500 — 800 Da) showed the highest inhibitory activity, and the relative inhibitory was 49.6%.
Oral administration of hydrolysate (3 ml, conc. = 3 g/kg B.W.) to spontaneously hypertensive rats (SHR) showed a hypotensive effect after 1 hour oral administration. The hydrolysate using Prozyme 6 hydrolysis for 5 hours showed maximal hypotensive effect at 1 hour after oral administration, and systolic blood pressure (SBP), diastolic blood pressure (DBP) and mean blood pressure (MBP) decreased by 18, 9 and 12 mmHg, respectively. The hydrolysate using Protease N for 5 hours showed the most potent hypotensive effect at 2 hour after oral administration, and systolic blood pressure (SBP), diastolic blood pressure (DBP) and mean blood pressure (MBP) decreased by 19, 20 and 19 mmHg, respectively. No relationship between heart beat and blood pressure was shown.
| Identifer | oai:union.ndltd.org:TW/090NTOU0253025 |
| Date | January 2002 |
| Creators | Yeh, Zheng Lin, 葉政霖 |
| Contributors | Tsai, Jenn Shou, 蔡震壽 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 72 |
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