碩士 / 國立海洋大學 / 食品科學系 / 90 / In search of the growth and the immunity promoting substance from food components. We used mammalian cell lines with serum-free medium under animal experiment model. We reported here with the growth and the immunity promoting substances from fish species. Fresh material were homogenized and then boiled at 100℃ for 30 mins. After centrifugation, the supernatant was further concentrated by vacuum evaporation. Through 30%-50% ammonium sulfate saturation precipitation (WG30-50). Those dialysis (ultrafiltration M.W.10 Da. cut off) were lyophilized. The result showed that extracts of Tilapia, milk fish enhanced cell proliferation toward human-human hybridoma cell line HB4C5 and enhanced cell proliferation toward human marrow cell lines HL60. Furtheμrmore, Taiwan small abalone, Halitotis diversicolor was selected for immunological function assessment. After small abalone was removed from the viscera, the pulp was treated by hot water (100℃, 30 mins). Hot-water extracts promoted cell proliferation toward human hybridoma cell line HB4C5, as well as macrophage-like cell lines (U937, UMf, THP-1 and J774.1) . We mixed Tilapia WGT3050, milk fish WGM3050 and small abalone, which show the immune bioactivity and named three-in-one. BALB/c mice were fed with different dose of three-in-one. One and two percent of mice daily eating were supplied by the three-in-one. Three-in-one significantly increased almost three-fold proliferation of spleen cell which is stimulating with LPS, either in specific or non-specific group is acceptable, and be stimulating with PHA in specific experiment. It increased one-fold to three-fold proliferation of PEC which is stimulating with PHA and LPS in specific or non-specific animal experiment. Two percent of mice daily eating is supplied by three-in-one also significantly increased in secretion of antibody IgG in specific or non-specific group. Both of the different dose were fed mouce also increased the ability of phagocytosis in whole blood. Through Phenyl column and Amicom ultrafiltration, we purify bioactive substances from WGT3050. These results obtained in this study indicated that the extract of three in one should be good candidate for health food development.
| Identifer | oai:union.ndltd.org:TW/090NTOU0253038 |
| Date | January 2002 |
| Creators | kuei-ying Lin, 林桂英 |
| Contributors | 龔瑞林 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 91 |
Page generated in 0.0012 seconds