碩士 / 國立海洋大學 / 食品科學系 / 90 / Abstract
Now, the kinds of cultured eels in Taiwan are mainly Anguilla japonica、A. anguilla and A. rostrata.The relationship of these three eels are familiar. When the processed products finished, it is difficult to identify the species of fish. The most favorite species of eel products is A. japonica. Because of lacking of A. japonica, the other two eel species are recommended to breed. On the other hand, the main conger eel in Taiwan is Muraensox cinereus, it is usually made to canned products. Most of three eel products are exported as processed eel products. The processed eel products haven’t any labels of species. To preclude false label of fish species, the gene identification of these four eel species has first established, and then the species of processed eel products collected from popular markets、wholesale markets and supermarkets were investigated. Application of polymerase chain reaction(PCR)- restriction fragment length polymorphism (RFLP) technology to quickly identify fish species was proved to be effective in this study.
In this study, the PCR technology and sequence analysis were needed eel to determine the amount of genetic variation in a 362 nucleotide region of the mitochondrial cytochrome b gene in fresh, frozen, steam sterilized meats of eels. These four eels had unique genotypes. The sequences were no difference between fresh, frozen and steam sterilized meats of eels. According to the data of genes, the three Anguilla species are familiar. PCR products of four eels were digested by using four restriction endonucleases. The highly conserved 362 base pair (bp) segment of mitochondrial cytochrome b gene was amplified and cleaved with restriction enzyme HinfI. The segment from A. japonica contained two restriction enzyme cutting sites; however, the segment from A. rostrata only had one site, and that from A. anguilla did not have any site.When restriction enzyme AseI cleaved, the segment from A. rostrata had one restriction enzyme cutting site, but A. japonica and A. anguilla had no site. Restriction enzyme Sau96I could differentiate eel species of M. cinereus from other three species of Anguilla.
To examine the species of twelve samples of commercial eel products, they were analyzed the cytochrome b gene fragment gene and the difference of restriction enzyme cutting site.The result showed that the species of commercial eel products included A. japonica、A. anguilla and A. rostrata. Nine out of twelve commercial eel products were A. japonica, one was A. rostrata, and the other two were A. anguilla. This indicates that the sequence and restriction enzyme cutting site analyses might be usable to authenticate species of different processed eel products.
| Identifer | oai:union.ndltd.org:TW/090NTOU0253048 |
| Date | January 2002 |
| Creators | JEN HSIAO CHIA, 任曉晶 |
| Contributors | Deng Fwu Hwang, 黃登福 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 88 |
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