碩士 / 東吳大學 / 微生物學系 / 90 / In Gordon-Kösswig Xiphophorus hybrid system, the black spots on the body of the purebred fish and hybrid offsprings show the same body-site-specific expression characteristics. Homeobox genes were understood as a major bodyplan ragulators. Many studies suggested that homeobox genes not only used as the signals as tissue-specific regulators but also played a role in the process of cell migration and localization. In order to understand the relationship between homeobox-containing genes and xiphophorine black spots body-site-specific expression, in this study, using a homeobox cloned from the xi- phophorine genome as a probe to screen the homeobox-containing fragments from the xiphophorine subgenomic libraries. At least 12 homeobox-containing fragments had been detected in the red swordtail fish genome after HindIII and EcoRI digestion. In order to isolate and identify these fragments, 10 subgenomic libraries were constructed. One clone had been selected. From sequence analysis, the clone contained only partial homeobox fragment. It might acquire some mutations and have frameshift within the homeodomain sequence during evolutional processes. Amplification of homeobox-containing fragments by PCR with specific primers from the ligation products for sub-libraries construction, 7 fragments had been amplified. After cloning and sequence analysis, the results showed that these fragments were mixtures. Further subcloning and sequence analysis of there fragments are processing.
| Identifer | oai:union.ndltd.org:TW/090SCU00381008 |
| Date | January 2002 |
| Creators | Ping-Chang Kuo, 郭秉昌 |
| Contributors | Kaun-Yu Lu, 路光予 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 0 |
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