碩士 / 台北醫學院 / 公共衛生學研究所 / 90 / A study was carried out to develop the extraction method and determine arsenic species in the main varieties of aquaculture seafood in blackfoot disease hyperendemic areas. Besides, the relationship between arsenic species of aquaculture ponds and marine arsenic species was explored. Biota samples were extracted with methanol/water (1/1) by Soxhlet extraction apparatus. The extracts were evaporated to dryness by cold evaporator, dissolved again in water, and filtered through C18 column. The concentrations of Arsenite, arsenate, monomethylarsonic acid (MMA), and dimethylarsinic acid (DMA) of extracts were measured by high performance liquid chromatography linked to hydride generator and atomic absorption spectrometry (HPLC-HG-AAS). Moreover, arsenobetaine was analyzed by HPLC-ultra violet (UV)-HG-AAS. The concentrations of arsenic species were determined in 71 mouthbreeder (oreochromis mossambicus) and 26 hard clam (meretrix lusoria) samples that were aquacultured in Putai, Ichu, Peimen, and Hsuehchia areas. Oyster (Crassotrea gigas) samples were collected from Putai, Anping, Hsiangshan, Tungshih, Tungkang, and Wangkung areas. The concentrations of arsenic species in biota samples and aquaculture pond water were analyzed. There were twenty-four percentage of aquaculture ponds arsenic concentrations higher than the standard of Drinking Water Act. The arsenic concentrations of Putai and Peimen pond water were higher than those of Ichu and Hsuehchia. Total arsenic and inorganic arsenic levels of aquaculture fish were lower than oyster and hard clam. Arsenite concentration in fish was significantly correlated with inorganic arsenic concentration in pond water. Arsenate level in fish was significantly correlated with the body length, width and weight of fish. Comparing the arsenic species with oyster, fish and hard clam, the inorganic percentage of total arsenic in oyster was lowest, and arsenobetaine percentage of total arsenic in fish was highest. Arsenite concentration of oyster collected from Putai was higher than that from Anping. Although fish and hard clam were collected from aquaculture ponds, the percentage of MMA and DMA relative to the sum of arsenic and its metabolites in fish was higher than in hard clam. The percentage of arsenite relative to the sum of arsenic and its metabolites in fish was lower than in hard clam. These results suggested that arsenic metabolic capability of fish was better than that of hard clam. The percentage of arsenite in bivalve, such as hard clam and oyster, relative to the sum of arsenic and its metabolites in hard clam was higher than that in oyster. It suggested that arsenic concentration of hard clam cultured in aquaculture ponds was higher than oyster bred in ocean. The inferior marine animals accumulate inorganic arsenic easily because their metabolism capabilities are fairly low.
| Identifer | oai:union.ndltd.org:TW/090TMC00058002 |
| Date | January 2002 |
| Creators | Huang Yung-Kai, 黃詠愷 |
| Contributors | Hsueh Yu-Mei, 薛玉梅 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 80 |
Page generated in 0.0112 seconds