碩士 / 國立陽明大學 / 解剖暨細胞生物學研究所 / 90 / ABSTRACT
Ursolic acid (UA), an active principle isolated from the Chinese herb “Souyang” (Cynomorium songaricum Rupr.), has been shown to the ability of antitumor and antiinflammation, and antioxidantion in liver cells. The effect of ursolic acid on excitotoxicity induced by kainate is still unknown. Primary neuronal cultures raised from cells isolated from the hippocampi of 7-day old rats were treated with 150 mM kainate. Our results revealed that 2 h of 150 mM kainate treatment led to significant generation of free radicals (224% relative to the control) and reduction of mitochondrial membrane potential (MMP) (71% relative to the control) and increase the accumulation of calcium ion in mitochondria (143% relative to the control). Ten minutes pretreatment with 5 - 15mM ursolic acid dose dependently and significantly attenuated the kainate-induced damage. Taken together, the results suggest that multiple mechanisms including protection of mitochondria, decrease in free radical generation, scavenging of free radicals and suppress the translation of GluR6, which reduce the calcium ion influx might be involved in ursolic acid’s protection against kainate induced cell toxicity.
| Identifer | oai:union.ndltd.org:TW/090YM000391011 |
| Date | January 2002 |
| Creators | Yi-Chun Chein, 簡溢均 |
| Contributors | Yu-Show Fu, 傅毓秀 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 66 |
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