碩士 / 國立陽明大學 / 遺傳學研究所 / 90 / The essential domains of human ribosomal protein L7 that involve in nuclear import, ribosome assembly, and in the function of protein synthesis are determined. L7 protein carries at least two nuclear localization signals (NLS): one locates at the N-region which carries several repeated NLS motifs, and the second one is tentatively identified at the middle section of protein. Either signal is capable of directing L7 into nucleus. Peptide contains the N-region of first 54 amino acid residues enters nuclearplasm and reaches nucleoli, whereas, truncated L7 proteins that either misses the N-region, the C-region of the last 50 amino acids, or both ends have condensed at nucleoli with little nuclearplasm retention. The C-region alone has a strong affinity to cytoplasmic membrane. When the assembly of ribosome was examined, all truncated proteins as long as having the middle section are incorporated into ribosome, and are capable of engaging in the protein synthesis.
Identifer | oai:union.ndltd.org:TW/090YM000498005 |
Date | January 2002 |
Creators | Chun-Lung Ko, 柯俊龍 |
Contributors | Alan Lin, 林茂榮 |
Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
Language | zh-TW |
Detected Language | English |
Type | 學位論文 ; thesis |
Format | 52 |
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