碩士 / 嘉南藥理科技大學 / 生物科技研究所 / 91 / In this study, a series of alginate/hydroxyapatite(HAP) composite scaffolds
were prepared and characterized. HAP was incorporated into the alginate gel
solution to improve the mechanical properties and biocompatibility of
scaffolds. The scaffold was prepared by a three-step procedure: first gelation
of the alginate solution to form a hydrogel with divalent cations (such as Ca2
＋, Sr2＋and Ba2＋), then freezing, and finally drying by lyophilization to
produce a three-dimensional, porous sponge. Alginate/HAP composite scaffolds
prepared in this paper not only have well interconnected, highly porous
structure but also have greater mechanical properties than the neat alginate
scaffold. The morphology of scaffolds can be manipulated by tuning the
quenching temperature during the preparation. The degradation behavior of
alginate/HAP composite scaffolds was easily controlled through the pretreatment
by immersing scaffolds into 1.0 M CaCl2 solution. Osteoblastic-like cell line,
UMR-106, seeded into the alginate/HAP composite scaffolds demonstrated better
biocompatibility than the pure one. These natural polymeric sponges fabricated
in this article may be a promising approach for tissue engineering applications
| Identifer | oai:union.ndltd.org:TW/091CNUPS111015 |
| Date | January 2003 |
| Creators | Yu-Jen Yeh, 葉郁仁 |
| Contributors | Hong-Ru Lin, 林鴻儒 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 78 |
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