碩士 / 國立屏東科技大學 / 水產養殖系 / 91 / The purpose of this research was to study the immunity of white shrimp, Litopenaeus vannamei. This study was carried out through three experiments. First part of the experiments was done by using different concentrations of immunostimulants of zymosan, phorbol myristate acetate (PMA), laminarin, sodium alginate and trypsin to induce the hemocytes of phenoloxidase (PO) and superoxidase anion (O2-) of the shrimps. The results showed that the PO and O2- in the hemocytes of white shrimp were induced some responses when sodium alginate, trypsin, and laminarin were used, whereas the prophenoloxidase activity was inhibited by the soybean trypsin inhibitor (STI) and benzamidine. The results also showed a prophenoloxidase system do exist in the hemocytes of white shrimp, but it is inactive. It showed that the three concentrations (75 U/μl, 150 U/μl, and 300 U/μl) of SOD were effective to clean the O2. in hemocytes of tested shrimp.
The second experiment was to test the PO activity, O2-, phagocytosis, clearance efficiency, and total count hemocytes (THC) were measured at the time intervals of 0, 30, 60, 90, and 120 minutes after using the sodium alginate to induce the factor of immunity and infecting by Vibrio damsela. The results showed that the PO activity and O2- were significantly increased (P<0.05), whereas the phagocytosis and clearance efficiency were significantly decreased (P<0.05). The THC was increased at first, but decreased afterwards.
The third experiment was to test the PO activity, O2-, phagocytosis, clearance efficiency, and total count hemocytes (THC) in hemocytes of white shrimp, and was measured at the time intervals of 0, 1, 2, and 4 weeks after feeding on algal powders, sodium alginate (500 ppm, 1000 ppm, and 2000 ppm), and an ordinary diet for 30 days. The results showed that the PO activity and O2- were significantly increased (P<0.05) after feeding with algal powders, and sodium alginate when compared with the control group. The results also showed that the immune disease-resistant ability of L. vannamei could be re-induced by injecting with white spot syndrome virus (WSSV) after the feeding treatments.
| Identifer | oai:union.ndltd.org:TW/091NPUST086003 |
| Date | January 2003 |
| Creators | Ya-Wen Hsiao, 蕭雅文 |
| Contributors | Po-Shing Chang, Win-Ton Cheng, 張朴性, 鄭文騰 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 106 |
Page generated in 0.0108 seconds